MYOPATHY IN EXPERIMENTAL MAGNESIUM DEFICIENCY *
MYOPATHY IN EXPERIMENTAL MAGNESIUM DEFICIENCY *
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实验性缺镁引起的肌病*
DOI：
10.1111/j.1749-6632.1969.tb13007.x
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发表时间：
1969
影响因子：
5.2
通讯作者：
H. Heggtveit
中科院分区：
文献类型：
--
作者：
H. Heggtveit
雌性Sprague-Dawley大鼠，体重约70 gm。用了由于生长需求增加和镁储存量减少，幼龄动物更容易受到镁耗竭的影响。实验组喂食标准化的镁缺乏饮食，每100 g中含有少于1 mg的镁。（General Biochemicals，Chagrin福尔斯，俄亥俄州）和去离子、双蒸馏饮用水（自由饮用）。g一个对照组接受上述饮食，每100克补充200 - 300毫克氯化镁，而第二组接受每100克含有200毫克镁的Purina食物。在第3、5、7、10和14天处死动物，此后每周处死一次，直至60天。将部分股前肌固定在中性福尔马林中并包埋在石蜡中用于光学显微镜检查。切片常规用苏木精和伊红（H&E）、高碘酸-希夫反应（PAS）和磷钨酸-苏木精（PTAH）染色。在钙化病例中使用von Kossa技术。在选定的大鼠中，用油红0对新鲜冷冻肌肉的低温恒温器切片进行染色以显示脂质。
Female Sprague-Dawley rats weighing approximately 70 gm. were used. The young animal is much more susceptible to the effects of Mg depletion because of increased growth requirements and smaller Mg stores. Experimental groups were fed a standardized, Mg-deficient diet containing less than 1 mg of Mg per 100 g. (General Biochemicals, Chagrin Falls, Ohio) and de-ionized, doubly-distilled drinking water ad libitum. A list of the components of this diet is published elsewhere.g One control group received the above diet supplemented with 200300 mg of Mg chloride per 100 gm, while a second got Purina chow containing 200 mg of Mg per 100 g. Animals were killed at 3, 5, 7, 10, and 14 days and at weekly intervals thereafter up to 60 days. Portions of anterior thigh muscles were fixed in neutral formalin and embedded in paraffin for light microscopy. Sections were stained routinely with hematoxylin and eosin (H&E) , periodic acid-Schiff reaction (PAS), and phosphotungstic acid-hematoxylin (PTAH) . The von Kossa technique was used in cases of calcification. In selected rats, cryostat sections of freshly frozen muscle were stained with Oil Red 0 to demonstrate lipid.