Dissecting the genetic architecture of Fusarium verticillioides seed rot resistance in maize by combining QTL mapping and genome-wide association analysis.
Dissecting the genetic architecture of Fusarium verticillioides seed rot resistance in maize by combining QTL mapping and genome-wide association analysis.
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DOI：
10.1038/srep46446
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发表时间：
2017-04-19
影响因子：
4.6
通讯作者：
Wu J
中科院分区：
文献类型：
--
作者：
Ju M;Zhou Z;Mu C;Zhang X;Gao J;Liang Y;Chen J;Wu Y;Li X;Wang S;Wen J;Yang L;Wu J
轮枝镰刀菌 (Fusarium verticillioides) 可通过种子传播并引起玉米全身感染 (Zea mays L.)；其霉菌毒素对动物和人类健康具有有害影响。我们将重组自交系 (RIL) 群体中的 QTL 作图与在受控条件下使用 224,152 个单核苷酸多态性 (SNP) 对 217 个不同玉米品系进行的全基因组关联研究 (GWAS) 相结合，以确定 F. verticillioides 种子腐烂 (FSR) 抗性的遗传结构。我们的研究分别通过连锁作图和 GWAS 鉴定了与 FSR 抗性相关的 8 个数量性状位点 (QTL) 和 43 个与 57 个 SNP 相关的基因。其中，有3个候选基因，即GRMZM2G0081223、AC213654.3_FG004和GRMZM2G099255，在连锁图谱和GWAS中均被检测到。此外，含有GRMZM2G0081223的近等基因系（NIL）也具有易感亲本背景，发现其抗性水平显着提高。此外，三个候选基因的表达谱表明，它们都对接种轮枝镰刀菌后的感染有反应。这些遗传分析表明，FSR抗性是由影响较小的位点控制的，具有有益等位基因和候选基因的品系的聚合育种可以提高玉米的FSR抗性。
Fusarium verticillioides can be transmitted via seeds and cause systemic infection in maize (Zea mays L.); its mycotoxin has harmful effects on animal and human health. We combined QTL mapping in recombinant inbred line (RIL) populations with a genome-wide association study (GWAS) of 217 diverse maize lines using 224,152 single nucleotide polymorphisms (SNPs) under controlled conditions to determine the genetic architecture of F. verticillioides seed rot (FSR) resistance. Our study identified 8 quantitative trait loci (QTLs) and 43 genes associated with 57 SNPs that were correlated with FSR resistance through linkage mapping and GWAS, respectively. Among these, there were three candidate genes, namely GRMZM2G0081223, AC213654.3_FG004, and GRMZM2G099255, which were detected in both linkage mapping and GWAS. Furthermore, the near-isogenic lines (NILs) containing GRMZM2G0081223, which also had a susceptible parent background, were found to have a significantly improved level of resistance. In addition, the expression profile of the three candidate genes revealed that they all respond to the infection following inoculation with F. verticillioides. These genetic analyses indicate that FSR resistance is controlled by loci with minor effect, and the polymerization breeding of lines with beneficial alleles and candidate genes could improve FSR resistance in maize.