KIF11 Is Required for Spheroid Formation by Oesophageal and Colorectal Cancer Cells
KIF11 Is Required for Spheroid Formation by Oesophageal and Colorectal Cancer Cells
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DOI：
10.21873/anticanres.11287
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发表时间：
2017-01-01
影响因子：
2
通讯作者：
Yasui, Wataru
中科院分区：
文献类型：
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作者：
Imai, Takeharu;Oue, Naohide;Yasui, Wataru
背景：食管鳞状细胞癌（ESCC）和结直肠癌（CRC）是人类常见的癌症类型。球形集落形成用于表征癌症干细胞（CSCs）。在本研究中，我们分析了激酶家族11 （KIF11）在人类ESCC和CRC中的意义。材料与方法：采用免疫组化方法检测105例ESCC和100例CRC中KIF11的表达。采用RNA干扰法抑制ESCC和CRC细胞系中KIF11的表达。结果：105例ESCC中有61例（58%），100例CRC中有62例（62%）KIF11阳性。KIF11的表达与任何临床病理特征无关。转染KIF11 siRNA后，TE-5 ESCC细胞和DLD-1 CRC细胞产生的球的数量和大小都比阴性对照siRNA转染显著减少。结论：这些结果提示KIF11在ESCC和CRC的CSCs中发挥重要作用。
Background: Oesophageal squamous cell carcinoma (ESCC) and colorectal cancer (CRC) are common types of human cancer. Spheroid colony formation is used to characterize cancer stem cell (CSCs). In the present study, we analyzed the significance of kinesin family 11 (KIF11 in human ESCC and CRC. Materials and Methods: Expression of KIF11 in 105 ESCC and 100 CRC cases was determined using immunohistochemistry. RNA interference was used to inhibit KIF11 expression in ESCC and CRC cell lines. Results: In total, 61 out of 105 (58%) ESCC and 62 out of 100 (62%) CRC cases were positive for KIF11. Expression of KIF11 was not associated with any clinicopathological characteristics. Both the number and size of spheres produced by from TE-5 ESCC cells and DLD-1 CRC cells were significantly reduced upon KIF11 siRNA transfection compared to negative control siRNA transfection. Conclusion: These results indicate that KIF11 plays an important role in CSCs of ESCC and CRC.