Site exclusion and sequence specificity in binding of 9-aminoacridine to the deoxytetranucleotide dpApGpCpT.
Site exclusion and sequence specificity in binding of 9-aminoacridine to the deoxytetranucleotide dpApGpCpT.
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9-氨基吖啶与脱氧四核苷酸 dpApGpCpT 结合的位点排除和序列特异性。
DOI：
10.1073/pnas.77.11.6453
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发表时间：
1980
影响因子：
11.1
通讯作者：
Kallenbach,NR
中科院分区：
文献类型：
--
作者：
Young,PR;Kallenbach,NR
用质子核磁共振滴定和热变性实验研究了诱变染料9-氨基吖啶（9AA）与自身互补四核苷酸dpApGpCpT的相互作用。在这个系统中，可以证明至少有三个明确定义的化学计量的复合物。复合物I是在过量四核苷酸存在下发生的1：2 9AA/四核苷酸结构。染料似乎嵌入四核苷酸双链体的GpC/GpC位点内。复合物II为2：2的9AA/四核苷酸结构，在ApG/CpT位点插入两个染料。复合物III是低温4：2 9AA/四核苷酸结构，除了存在于复合物II中的那些之外，还含有堆叠在双链体的末端A-T残基上的两个染料分子。这些结果表明，序列选择性和位点排斥都可以发生在这个模型系统。
The interaction of the mutagenic dye 9-aminoacridine (9AA) with the self-complementary tetranucleotide dpApGpCpT has been studied by a combination of proton NMR titrations and thermal denaturation experiments. A minimum of three complexes of well-defined stoichiometry can be demonstrated in this system. Complex I is a 1:2 9AA/tetranucleotide structure occurring in the presence of excess tetranucleotide. The dye appears to intercalate within the GpC/GpC site of a tetranucleotide duplex. Complex II is a 2:2 9AA/tetranucleotide structure, with two dyes intercalated at the ApG/CpT sites of the duplex. Complex III is a low-temperature 4:2 9AA/tetranucleotide structure containing two dye molecules stacked over the terminal A-T residues of the duplex in addition to those present in complex II. These results show that both sequence selectivity and site exclusion can occur in this model system.