Optimizing Phosphoric Acid plus Hydrogen Peroxide (PHP) Pretreatment on Wheat Straw by Response Surface Method for Enzymatic Saccharification
Optimizing Phosphoric Acid plus Hydrogen Peroxide (PHP) Pretreatment on Wheat Straw by Response Surface Method for Enzymatic Saccharification
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DOI：
10.1007/s12010-016-2273-7
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发表时间：
2017-03-01
影响因子：
3
通讯作者：
Song, Chun
中科院分区：
文献类型：
--
作者：
Qiu, Jingwen;Wang, Qing;Song, Chun
采用磷酸加过氧化氢（PHP）对麦秸进行预处理，利用响应面法考察了预处理温度、时间和H3PO4配比。结果表明，预处理温度、H3PO4比例和时间的增加对半纤维素和木质素的去除率均有正向响应。H3PO4比例是控制纤维素回收率的最重要变量，其次是预处理温度和预处理时间。此外，这三个变量均与纤维素回收率负相关。增加H3PO4的比例可以改善酶解；然而，纤维素回收率的降低导致了葡萄糖产率的降低。过高的预处理温度或过长的预处理时间不利于酶解和葡萄糖得率。以H3PO4用量最少、葡萄糖产率最高为标准，优化预处理条件为：40℃、2.0 h、H3PO4用量为70.2% (H2O2用量为5.2%)，酶解72 h后，葡萄糖产率为299 mg/g麦秸（纤维素产率为946.2 mg/g）。
Wheat straw was pretreated by phosphoric acid plus hydrogen peroxide (PHP), in which temperature, time, and H3PO4 proportion for pretreatment were investigated by using response surface method. Results indicated that hemicellulose and lignin removal positively responded to the increase of pretreatment temperature, H3PO4 proportion, and time. H3PO4 proportion was the most important variable to control cellulose recovery, followed by pretreatment temperature and time. Moreover, these three variables all negatively related to cellulose recovery. Increasing H3PO4 proportion can improve enzymatic hydrolysis; however, reduction on cellulose recovery results in decrease of glucose yield. Extra high temperature or long time for pretreatment was not beneficial to enzymatic hydrolysis and glucose yield. Based on the criterion for minimizing H3PO4 usage and maximizing glucose yield, the optimized pretreatment conditions was 40 A degrees C, 2.0 h, and H3PO4 proportion of 70.2 % (H2O2 proportion of 5.2 %), by which glucose yielded 299 mg/g wheat straw (946.2 mg/g cellulose) after 72-h enzymatic hydrolysis.