Microspheres of Carboxymethyl Chitosan, Sodium Alginate, and Collagen as a Hemostatic Agent in Vivo
Microspheres of Carboxymethyl Chitosan, Sodium Alginate, and Collagen as a Hemostatic Agent in Vivo
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DOI：
10.1021/acsbiomaterials.8b00453
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发表时间：
2018-07-01
影响因子：
5.8
通讯作者：
Lv, Zhengbing
中科院分区：
文献类型：
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作者：
Jin, Jia;Ji, Zhixiao;Lv, Zhengbing
在寻找生物相容性复合微球作为止血剂的研究中，在先前的研究中，我们设计了一种新型的生物材料，由含有三种天然生物成分的复合微球组成，羧甲基壳聚糖，海藻酸钠和胶原蛋白（CSCM）。并对其理化性能、止血性能、生物相容性和细胞毒性进行了体外研究。在这项工作中，在体内止血性能，伤口愈合，血液相容性，组织相容性和生物降解性进行了评价，通过一系列的实验。结果表明，CSCM可以通过加速凝血和伤口闭合速度来止血和提高愈合效率，表明CSCM具有止血作用，并促进伤口愈合。此外，CSCM材料的皮内刺激反应可忽略不计，无明显溶血反应。更重要的是，CSCM可以在体内降解，而不会对生理，生物化学和组织产生重大影响。因此，CSCM可能是一个有用的工具，止血在紧急情况下，在军事和民用设置。
In the search for biocompatible composite microspheres to be used as a hemostatic agent, in a previous study, we designed a novel biomaterial, consisting of composite microspheres containing three natural biological ingredients, carboxymethyl chitosan, sodium alginate and collagen (CSCM). Furthermore, the chemical and physical properties, hemostatic ability, biocompatibility and cytotoxicity were investigated in vitro. In this work, the in vivo hemostatic performance, wound healing, hemocompatibility, histocompatibility, and biodegradability were evaluated by a series of experiments. The results showed that CSCM could both stop bleeding and enhance healing efficiency by accelerating the clotting and the wound closure rate, suggesting that CSCM acts as a hemostat, and enhances wound healing. In addition, the CSCM material had negligible intracutaneous stimulation reactions and no obvious hemolytic reactions. More importantly, CSCM can be degraded in vivo without significant impacts on physiology, biochemistry, and organization. Thus, CSCM may be a useful tool to stop bleeding in emergency conditions in both military and civilian settings.