Microfluidic electrochemical sensor for on-line monitoring of aerosol oxidative activity.
Microfluidic electrochemical sensor for on-line monitoring of aerosol oxidative activity.
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DOI：
10.1021/ja3031104
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发表时间：
2012-06-27
影响因子：
15
通讯作者：
Henry, Charles S.
中科院分区：
文献类型：
--
作者：
Sameenoi, Yupaporn;Koehler, Kirsten;Shapiro, Jeff;Boonsong, Kanokporn;Sun, Yele;Collett, Jeffrey, Jr.;Volckens, John;Henry, Charles S.
颗粒物（PM）空气污染对人类发病率和死亡率有重大影响；然而，pm诱导的毒性机制尚不明确。一个主要的假设认为，空气中的PM通过在人体组织内部和周围产生活性氧（ROS）而引起氧化应激，从而造成伤害。我们在这里报告了一种采用微流体电化学传感器直接耦合到颗粒进液采样器（PILS）系统的系统，以在线形式测量气溶胶氧化活性。氧化活性测量是基于二硫代苏糖醇测定法（DTT测定法），在被PM氧化后，剩余的还原DTT通过微流控传感器进行分析。该传感器由一组工作电极、参考电极和辅助电极组成，这些电极是用聚二甲基硅氧烷（PDMS）为基础的微流体装置制造的。采用钴（II）酞菁（CoPC）改性碳糊作为工作电极材料，选择性检测还原DTT。利用从城市环境和生物质燃烧事件中采集的过滤样品，离线验证了电化学传感器与传统DTT分析的对比。离线表征后，将传感器与PILS耦合，以实现气溶胶氧化活性的在线采样/分析。城市粉尘和工业焚化炉灰样品在气溶胶室中雾化并分析其氧化活性。在线传感器报告DTT消耗率（氧化活性）与气溶胶浓度有良好的相关性（R2从0.86 -）。97)，时间分辨率约为3分钟。
Particulate matter (PM) air pollution has a significant impact on human morbidity and mortality; however, the mechanisms of PM-induced toxicity are poorly defined. A leading hypothesis states that airborne PM induces harm by generating reactive oxygen species (ROS) in and around human tissues, leading to oxidative stress. We report here, a system employing a microfluidic electrochemical sensor coupled directly to a Particle-into-Liquid-Sampler (PILS) system to measure aerosol oxidative activity in an on-line format. The oxidative activity measurement is based on the dithiothreitol assay (DTT assay) where after oxidized by PM, the remaining reduced DTT was analyzed by the microfluidic sensor. The sensor consists of an array of working, reference, and auxiliary electrodes fabricated in a poly(dimethylsiloxane) (PDMS)-based microfluidic device. Cobalt (II) phthalocyanine (CoPC)-modified carbon paste was used as the working electrode material allowing selective detection of reduced DTT. The electrochemical sensor was validated off-line against the traditional DTT assay using filter samples taken from urban environments and biomass burning events. After off-line characterization, the sensor was coupled to a PILS to enable on-line sampling/analysis of aerosol oxidative activity. Urban dust and industrial incinerator ash samples were aerosolized in an aerosol chamber and analyzed for their oxidative activity. The on-line sensor reported DTT consumption rates (oxidative activity) in good correlation with aerosol concentration (R2 from 0.86–.97) with a time-resolution of approximately 3 minutes.
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影响因子：
5
作者：
Jung, H;Guo, B;Kennedy, IM
通讯作者：
Kennedy, IM
影响因子：
4.2
作者：
COOKEAS, EG;EFSTATHIOU, CE
通讯作者：
EFSTATHIOU, CE
影响因子：
7.4
作者：
HALBERT, MK;BALDWIN, RP
通讯作者：
BALDWIN, RP
影响因子：
3.8
作者：
Ghelfi, Elisa;Ramos-Rhoden, Claudia;Gonzalez-Flecha, Beatriz
通讯作者：
Gonzalez-Flecha, Beatriz
影响因子：
2.1
作者：
Clarke, RW;Catalano, P;Godleski, JJ
通讯作者：
Godleski, JJ