Developing japonica rice introgression lines with multiple resistance genes for brown planthopper, bacterial blight, rice blast, and rice stripe virus using molecular breeding
Developing japonica rice introgression lines with multiple resistance genes for brown planthopper, bacterial blight, rice blast, and rice stripe virus using molecular breeding
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DOI：
10.1007/s00438-018-1470-1
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发表时间：
2018-12-01
影响因子：
3.1
通讯作者：
Kim, Bo-Kyeong
中科院分区：
文献类型：
--
作者：
Reinke, Russell;Kim, Suk-Man;Kim, Bo-Kyeong
由于真菌、细菌、病毒和昆虫的生物胁迫而造成的产量损失是大多数水稻种植区面临的一个关键挑战。培育抗病品种被认为是缓解水稻减产的有效和可持续的途径。在本研究中，我们描述了发展的粳稻渐渗系与多个抗性基因（MR线），耐四种不同类型的生物胁迫，并比较这些线的农艺性状，产量和粮食品质参数与轮回亲本。共9个MR线开发的标记辅助回交，其中结合了5个单R基因在粳稻背景与最小的连锁阻力。所有的MR系都含有R基因Bph18和qSTV11（SG）以及两个共同的Pi基因（Pib+Pik），分别对褐飞虱（BPH）、水稻条纹病毒（RSV）和稻瘟病具有抗性。在细菌性枯萎病（BB）的情况下，Xa40仅在9个中的5个中被检测到，Xa3在其他中被验证。特别地，与Xa40组合的五个转R基因的MR系（Bph18+qSTV11SG+Pib+Pik）对BPH、BB、blast和RSV的所有生物测定显示稳定的抗性。这些不育系对水稻的主要农艺性状，包括产量和稻米品质没有表现出任何负面影响。这些品系具有稳定水稻产量和最大限度地降低韩国病虫害多发地区生产成本的巨大潜力，通过使用标记辅助回交策略对5个R基因进行杂交。
Yield losses as a result of biotic stresses by fungi, bacteria, viruses, and insects are a key challenge in most rice cultivation areas. The development of resistant cultivars is considered an efficient and sustainable approach to mitigate rice yield reduction. In the present study, we describe the development of japonica rice introgression lines with multiple resistance genes (MR lines), resistant to four different types of biotic stresses, and compare the agronomic performance, yield, and grain quality parameters of these lines with those of the recurrent parent. A total of nine MR lines were developed by marker-assisted backcrossing, which combined five single-R genes in a japonica background with a minimum of linkage drag. All the MR lines harbored the R genes Bph18 and qSTV11(SG) and two Pi genes (Pib+Pik) in common, offering resistance to brown planthopper (BPH), rice stripe virus (RSV), and rice blast disease, respectively. In the case of bacterial blight (BB), Xa40 was detected in only five out of the nine and Xa3 was validated in the others. In particular, the five MR lines pyramiding the R genes (Bph18+qSTV11SG+Pib+Pik) in combination with Xa40 showed stable resistance to all bioassays for BPH, BB, blast, and RSV. The MR lines did not show any negative effects on the main agronomic traits, including yield production and rice grain quality. The lines have significant potential to stabilize rice yield and minimize production costs in disease and pest-prone areas in Korea, through the pyramiding of five R genes using a marker-assisted backcrossing strategy.