Fluorescent probe for copper(II) ion based on a rhodamine spirolactame derivative, and its application to fluorescent imaging in living cells
Fluorescent probe for copper(II) ion based on a rhodamine spirolactame derivative, and its application to fluorescent imaging in living cells
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DOI：
10.1007/s00604-011-0623-2
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发表时间：
2011-09-01
影响因子：
5.7
通讯作者：
Chen, Lingxin
中科院分区：
文献类型：
--
作者：
Yu, Chunwei;Zhang, Jun;Chen, Lingxin
A fluorescent probe for Cu(II) ion is presented. It is based on the rhodamine fluorophore and exhibits high selectivity and sensitivity for Cu(II) ion in aqueous methanol (2:8, v/v) at pH 7.0. The response is based on a ring opening reaction and formation of a strongly fluorescent 1:1 complex. The response is reversible and linear in the range between 50 nM and 900 nM, with a detection limit of 7.0 nM. The probe was successfully applied to fluorescent imaging of Cu(II) ions in HeLa cells.
