Complete angle small animal fluorescence imaging with early-arriving photons.
Complete angle small animal fluorescence imaging with early-arriving photons.
复制标题
使用提前到达的光子进行全角度小动物荧光成像。
DOI：
10.1109/iembs.2009.5333167
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发表时间：
2009
期刊：
影响因子：
--
通讯作者：
Ntziachristos,Vasilis
中科院分区：
文献类型：
--
作者：
Niedre,Mark;Ntziachristos,Vasilis
荧光介导的断层扫描允许在整个动物中对光学报告探针进行定量的三维成像，因此正在成为一种强大的分子成像工具。荧光断层摄影术中可实现的图像质量受到生物组织中高度光散射的限制。时间门控检测早期到达的，因此最小散射的光子通过扩散组织传输是一种策略，用于最大限度地减少光散射的影响。在这项工作中，我们进行了全角度断层成像的小鼠植入荧光管使用时间门控检测早期和晚期到达的光子。这是使用飞秒激光和高速，时间选通增强CCD成像仪实现的。我们证明，早期传输的荧光光子允许改善荧光分布的可视化，即使考虑通过动物的个人投影。高保真图像重建使用72个投影在5度的步骤，使用早期到达的光子也被证明。
Fluorescence mediated tomography allows quantitative, three-dimensional imaging of optical reporter probes in whole animals and is therefore emerging as a powerful molecular imaging tool. The achievable image quality in fluorescence tomography is limited by the high-degree of light scatter in biological tissue. Time-gated detection of early-arriving and therefore minimally-scattered photons transmitted through diffusive tissue is one strategy for minimizing the effects of light scatter. In this work, we performed full-angle tomographic imaging of mice implanted with fluorescent tubes using time-gated detection of early- and later-arriving photons. This was achieved using a femtosecond laser and a high-speed, time-gated intensified CCD imager. We demonstrate that the early-transmitted fluorescent photons allow improved visualization of the fluorescence distribution, even when considering individual projections through the animal. High-fidelity image reconstruction using 72 projections in 5-degree steps using early-arriving photons is also demonstrated.