Chromosome-level assembly of Culex pipiens molestus and improved reference genome of Culex pipiens pallens (Culicidae, Diptera)
Chromosome-level assembly of Culex pipiens molestus and improved reference genome of Culex pipiens pallens (Culicidae, Diptera)
复制标题
淡色库蚊的染色体水平组装和淡色库蚊（库蚊科，双翅目）的改进参考基因组
DOI：
10.1111/1755-0998.13712
复制
发表时间：
2022-09-18
影响因子：
7.7
通讯作者：
Zhang, Ruiling
中科院分区：
文献类型：
--
作者：
Liu, Wenjuan;Cheng, Peng;Zhang, Ruiling
淡色库蚊和淡色库蚊是淡色库蚊复合体中两种截然不同的生物形态，是多种病原体的重要媒介，广泛分布于世界各地。在目前的研究中，我们展示了一个高质量的CX染色体水平的基因组。并描述了该基因组的遗传特征。组装基因组全长559.749 Mb，重叠群和骨架N50分别为200.952 Mb和0.370 Mb，94.78%以上的组装碱基位于3条染色体上。总共预测了19399个蛋白质编码基因。Cx基因组中扩增出多个基因家族。尤其是化学感觉蛋白(CSP)和味觉受体(GR)基因家族的成员。此外，利用Hi-C数据，我们对先前组装的Cx基因组草稿进行了改进。Piniens F.pallens，其骨架N50为186.195 Mb，重叠群N50为0.749 Mb，97.02%以上的组装碱基位于3条染色体上。该参考基因组为基于基因组的Cx独特的生态和进化特征的研究提供了基础。以及本研究的结果将有助于阐明库蚊复合体物种分化的机制。
Culex pipiens molestus and Culex pipiens pallens are two distinct bioforms in the Culex pipiens complex that are important vectors of several pathogens and are widely distributed around the world. In the current study, we present a high-quality chromosome-level genome of Cx. pipiens f. molestus and describe the genetic characteristics of this genome. The assembly genome was 559.749 Mb with contig and scaffold N50 values of 200.952 Mb and 0.370 Mb, and more than 94.78% of the assembled bases were located on 3 chromosomes. A total of 19,399 protein-coding genes were predicted. Many gene families were expanded in the genome of Cx. pipiens f. molestus, particularly those of the chemosensory protein (CSP) and gustatory receptor (GR) gene families. In addition, utilizing Hi-C data, we improved the previously assembled draft genome of Cx. pipiens f. pallens, with scaffold N50 of 186.195 Mb and contig N50 of 0.749 Mb, and more than 97.02% of the assembled bases were located on three chromosomes. This reference genome provides a foundation for genome-based investigations of the unique ecological and evolutionary characteristics of Cx. pipiens f. molestus, and the findings in this study will help to elucidate the mechanisms involved in species divergence in the Culex pipiens complex.