Inhibitory effects of glutathione on dengue virus production.
Inhibitory effects of glutathione on dengue virus production.
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DOI：
10.1016/j.bbrc.2010.05.108
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发表时间：
2010-07
影响因子：
3.1
通讯作者：
Yanping Tian;Wen Jiang;Na Gao;Jun-lei Zhang;Wei Chen;Dong-ying Fan;De‐shan Zhou;J. An
中科院分区：
文献类型：
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作者：
Yanping Tian;Wen Jiang;Na Gao;Jun-lei Zhang;Wei Chen;Dong-ying Fan;De‐shan Zhou;J. An
还原型谷胱甘肽(GSH)是最有效的细胞内抗氧化剂，也与病毒感染有关。登革病毒(DV)感染的发病机制尚未完全阐明。本研究探讨登革病毒2型(DV2)感染与宿主细胞内GSH含量的关系。结果表明，DV2感染细胞后，细胞内谷胱甘肽含量降低，导致NF-κB活化，DV2产量增加。补充谷胱甘肽显著抑制了NF-κB的激活，导致细胞中DV2的产生减少。此外，经谷胱甘肽合成抑制剂丁硫氨酸亚磺胺处理的肝癌细胞中，NF-κB活性升高，DV2产量增加。综上所述，DV2感染可降低宿主细胞内GSH浓度，并从这一过程中受益。补充GSH可抑制病毒的产生，提示GSH在预防和治疗DV2感染方面有一定的应用价值。
Reduced glutathione (GSH) is the most powerful intracellular antioxidant and also involved in viral infections. The pathogenesis of dengue virus (DV) infection has not been completely clarified. This study investigated the relationship between DV serotype 2 (DV2) infections and host intracellular GSH content. Results showed infection with DV2 resulted in a decrease in intracellular GSH, which caused NF-κB activation and increased DV2 production. Supplemental GSH significantly inhibited activation of NF-κB, resulting in a decreased production of DV2 in HepG2 cells. Furthermore, high activity of NF-κB and increased production of DV2 was observed in HepG2 cells treated with buthionine sulfoximine (BSO), an inhibitor of GSH synthesis. In conclusion, DV2 infection could reduce host intracellular GSH concentration and benefited from this process. Supplemental GSH could inhibit viral production, indicating GSH might be valuable in the prevention and treatment of DV2 infection.