Synonymous nucleotide substitution rates of beta-tubulin and histone genes conform to high overall genomic rates in rodents but not in sea urchins.
Synonymous nucleotide substitution rates of beta-tubulin and histone genes conform to high overall genomic rates in rodents but not in sea urchins.
复制标题
β-微管蛋白和组蛋白基因的同义核苷酸取代率符合啮齿类动物的高整体基因组率，但不符合海胆的高整体基因组率。
DOI：
10.1007/bf02099730
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发表时间：
1988
影响因子：
3.9
通讯作者：
Nemer,M
中科院分区：
文献类型：
--
作者：
Harlow,P;Litwin,S;Nemer,M
海胆和啮齿动物的基因组进化迅速，单拷贝核DNA序列的差异表明了这一点。我们通过比较两种海胆β-Tubuin、组蛋白H4和组蛋白H3以及啮齿动物和人类之间的序列，检验了三个高度保守的基因-微管蛋白、组蛋白H4和组蛋白H3的同义替换率是否与这些高基因组替换率保持一致。而β-微管蛋白基因3‘端非翻译区之间的变化率。PurPuratus(Sp-β1)和Ofl.Pictus(LP-β3)与这些物种之间的杂交结果估计的总体变化率是一致的，这些β-微管蛋白的羧基结构域以及晚期组蛋白H4和H3的同义替换率显著降低。相反，啮齿动物之间以及啮齿动物和人类之间对于相同β-微管蛋白同型的羧基结构域本身以及组蛋白H4和H3.1的同义核苷酸替换率与啮齿动物基因组的总体变化率没有不同。此外，对同源的人和小鼠的β-微管蛋白序列的分析支持这样的结论，即小鼠的同义替换率高于人类。严格地从这些基因保守的氨基酸序列和碱基组成来看，对进化变化的制约差异并不明显。其他限制性影响似乎更多地与海胆β-微管蛋白和组蛋白编码区的同义替换率偏离平均基因组速率有关。
Sea urchin and rodent genomes have been posited to evolve rapidly as indicated by divergences in single copy nuclear DNA sequences. We have examined whether the synonymous substitution rates of three highly conserved genes, β-tubulin, histone H4, and histone H3, adhere to these high genomic substitution rates by comparing sequences between two sea urchins,Strongylocentrotus purpuratusandLytechinus pictus, and between rodents and humans. Whereas the rate of change between the 3′ untranslated regions of the β-tubulin cDNA ofS. purpuratus(Sp-β1), sequenced in this study, and ofL. pictus(Lp-β3) was consistent with the overall rate of change estimated from previous DNA hybridization results between these species, the synonymous substitution rates for the carboxyl domains of these β-tubulins, as well as for the late histones H4 and H3, were significantly depressed. In contrast, synonymous nucleotide substitution rates between rodents and between rodent and human for the carboxyl domain proper of identical β-tubulin isotypes and for histone H4 and H3.1 did not differ from the overall rate of change for the rodent genomes. Moreover, an analysis of paralogous human and mouse β-tubulin sequences supported the conclusion that the synonymous substitution rates in the mouse were higher than those in the human. Differences in constraint on evolutionary change were not evident strictly from the conserved amino acid sequences and base compositions of these genes. Other constraining influences seemed more relevant to the departure of the synonymous substitution rates of the sea urchin β-tubulin and histone coding regions from the average genomic rate.