NeuroD1: developmental expression and regulated genes in the rodent pineal gland.
NeuroD1: developmental expression and regulated genes in the rodent pineal gland.
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NeuroD1：啮齿动物松果体中的发育表达和调节基因。
DOI：
10.1111/j.1471-4159.2007.04605.x
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发表时间：
2007
影响因子：
4.7
通讯作者：
Klein,DavidC
中科院分区：
文献类型：
--
作者：
Munoz,EstelaM;Bailey,MichaelJ;Rath,MartinF;Shi,Qiong;Morin,Fabrice;Coon,StevenL;Moller,Morten;Klein,DavidC
NeuroD 1/BETA2是bHLH转录因子家族的成员，已知其影响特定神经元、内分泌和视网膜细胞的命运。我们在这里报告，NeuroD 1 mRNA是高度丰富的发展和成年大鼠松果体。松果体的表达开始于17天的胚胎，此时它也可以在其他大脑区域中检测到。松果体中的表达在胚胎期增加，此后维持在与小脑和视网膜中发现的水平相当的水平。相比之下，NeuroD 1 mRNA在发育过程中在非小脑脑区显着减少。松果体NeuroD 1水平在白天和晚上是相似的，并且似乎不受交感神经输入的影响。对来自神经元D1基因敲除小鼠的松果体的基因表达分析鉴定出127个下调的转录物（>两倍，p<0.05）和16个上调的转录物（>两倍，p<0.05）。 根据定量RT-PCR，最显著下调的基因是驱动蛋白家族成员5C（约100倍），最显著上调的基因是谷氨酸脱羧酶1（约104倍）。其他受影响的转录本编码参与分化、发育、信号转导和运输的蛋白质。这些发现代表了阐明NeuroD 1在啮齿动物松果体细胞中作用的第一步。
NeuroD1/BETA2, a member of the bHLH transcription factor family, is known to influence the fate of specific neuronal, endocrine and retinal cells. We report here that NeuroD1 mRNA is highly abundant in the developing and adult rat pineal gland. Pineal expression begins in the 17‐day embryo at which time it is also detectable in other brain regions. Expression in the pineal gland increases during the embryonic period and is maintained thereafter at levels equivalent to those found in the cerebellum and retina. In contrast, NeuroD1 mRNA decreases markedly in non‐cerebellar brain regions during development. Pineal NeuroD1 levels are similar during the day and night, and do not appear to be influenced by sympathetic neural input. Gene expression analysis of the pineal glands from neonatalNeuroD1knockout mice identifies 127 transcripts that are down‐regulated (>twofold,p< 0.05) and 16 that are up‐regulated (>twofold,p< 0.05). According to quantitative RT‐PCR, the most dramatically down‐regulated gene is kinesin family member 5C (∼100‐fold) and the most dramatically up‐regulated gene is glutamic acid decarboxylase 1 (∼fourfold). Other impacted transcripts encode proteins involved in differentiation, development, signal transduction and trafficking. These findings represent the first step toward elucidating the role ofNeuroD1in the rodent pinealocyte.