Blood flow rate and energy charge in the isolated perfused canine liver.
Blood flow rate and energy charge in the isolated perfused canine liver.
复制标题
离体灌注犬肝脏中的血流量和能量电荷。
DOI：
10.1006/jsre.2001.6102
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发表时间：
2001
期刊：
影响因子：
--
通讯作者：
C. Leal
中科院分区：
文献类型：
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作者：
S. Rodríguez;E. Portilla;D. García;F. Larios;M. Romero;J. Muñiz;G. Vázquez;C. Leal
背景。肝脏活力取决于血流量，不同研究中使用的离体灌注肝脏之间的血流量有所不同。我们的目的是确定能够保持能量负荷与肝脏活力相一致的最小血流量。材料和方法。将离体犬肝脏分配至不同组（n = 4），血流量分别为 0.5（组 I）、1.0（组 II）和 1.5 ml/g/min（组 III）。在 180 分钟内测量血气、血细胞比容和血糖。还测量了核苷酸、血清渗透压和电解质。在每次实验结束时记录形态变化。结果。第三组肝脏的酸碱平衡更好。电解质、渗透压、血细胞比容和葡萄糖没有显示差异。较高的能量消耗与较高的血流速率相关。在组织病理学方面，有证据表明第三组有更多与静水压相关的变化，而坏死或炎症相关的改变没有差异。结论。 (1) 能量电荷与血流速率密切相关。 (2) 1.5 ml/g/min 的速率可实现更好的电解质、渗透压和酸碱平衡。 (3) 形态学分析并不是通过能量势评估的器官活力的敏感预测因子。 (4) 获得高于 0.75 的能量电荷所需的最小血流量为 1.33 ml/g/min。
Background. Liver viability depends on blood flow rate, which varies among isolated perfused livers used in different studies. We aimed to identify the minimal blood flow capable of keeping the energy charge compatible with liver viability. Materials and methods. Isolated canine livers were assigned to different groups (n = 4) with blood flow rates of 0.5 (Group I), 1.0 (Group II), and 1.5 ml/g/min (Group III). Blood gases, hematocrit, and glucose were measured over 180 min. Nucleotides, serum osmolarity, and electrolytes were also measured. Morphological changes were recorded at the end of each experiment. Results. Acid-base balance was better in livers from Group III. Electrolytes, osmolarity, hematocrit, and glucose showed no differences. Higher energy charges correlated with higher blood flow rates. On histopathology, there was evidence of more hydrostatic pressure-related changes in Group III, with no difference in necrosis or inflammatory-related alterations. Conclusions. (1) Energy charge correlates strongly with blood flow rate. (2) A rate of 1.5 ml/g/min allows for a better electrolyte, osmolarity, and acid-base balance. (3) Morphological analysis is not a sensitive predictor of organ viability as assessed by energetic potential. (4) The minimal blood flow rate required to attain an energy charge above 0.75 is 1.33 ml/g/min.