Transfection of the IHH gene into rabbit BMSCs in a simulated microgravity environment promotes chondrogenic differentiation and inhibits cartilage aging.
Transfection of the IHH gene into rabbit BMSCs in a simulated microgravity environment promotes chondrogenic differentiation and inhibits cartilage aging.
复制标题
模拟微重力环境下将IHH基因转染兔BMSCs促进软骨分化并抑制软骨衰老
DOI：
10.18632/oncotarget.11871
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发表时间：
2016-09-27
期刊：
影响因子：
--
通讯作者：
Wu X
中科院分区：
文献类型：
--
作者：
Liu PC;Liu K;Liu JF;Xia K;Chen LY;Wu X
在模拟微重力环境下，研究过表达印度刺猬（Indianhedgehog，IHH）基因对兔骨髓间充质干细胞（BMSCs）向软骨细胞分化的影响。体外构建兔IHH基因的腺病毒质粒，转染兔BMSCs。实验分为两大组：常规细胞培养及诱导模型组和模拟微重力环境组。每个大组又分为空白对照组、GFP转染组和IHH转染组。在分化诱导过程中，测定各组软骨相关和软骨肥大相关基因和蛋白的表达水平。在常规模型中，IHH转染组在分化诱导的早期表达高水平的软骨相关因子（Coll2和ANCN），并且在晚期表达高水平的软骨肥大相关因子（Coll10、膜联蛋白5和ALP）。在模拟微重力环境下，IHH转染组在分化诱导的各个阶段表达高水平的软骨相关因子和低水平的软骨肥大相关因子。在模拟微重力环境下，转染IHH基因的BMSCs能有效促进软骨的生成，抑制软骨老化和成骨。因此，该技术适用于软骨组织工程。
The effect of overexpressing the Indian hedgehog (IHH) gene on the chondrogenic differentiation of rabbit bone marrow-derived mesenchymal stem cells (BMSCs) was investigated in a simulated microgravity environment. An adenovirus plasmid encoding the rabbit IHH gene was constructed in vitro and transfected into rabbit BMSCs. Two large groups were used: conventional cell culture and induction model group and simulated microgravity environment group. Each large group was further divided into blank control group, GFP transfection group, and IHH transfection group. During differentiation induction, the expression levels of cartilage-related and cartilage hypertrophy-related genes and proteins in each group were determined. In the conventional model, the IHH transfection group expressed high levels of cartilage-related factors (Coll2 and ANCN) at the early stage of differentiation induction and expressed high levels of cartilage hypertrophy-related factors (Coll10, annexin 5, and ALP) at the late stage. Under the simulated microgravity environment, the IHH transfection group expressed high levels of cartilage-related factors and low levels of cartilage hypertrophy-related factors at all stages of differentiation induction. Under the simulated microgravity environment, transfection of the IHH gene into BMSCs effectively promoted the generation of cartilage and inhibited cartilage aging and osteogenesis. Therefore, this technique is suitable for cartilage tissue engineering.