Presence of auto-anti-idiotypic antibody during the normal human immune response to tetanus toxoid antigen.
Presence of auto-anti-idiotypic antibody during the normal human immune response to tetanus toxoid antigen.
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在正常人体对破伤风类毒素抗原的免疫反应过程中存在自身抗独特型抗体。
作者：
R. Geha
在接种破伤风类毒素（TT）抗原加强免疫的两例患者血清中寻找自身抗独特型抗体。从免疫后4个月的连续出血中获得的IgG进行了研究，a)其特异性结合125I IgG F(ab')2抗TT的能力，从加强免疫后7和10天获得的血清中制备，b)其特异性抑制125I放射性标记TT与免疫后7和10天获得的IgG结合的能力，并指定为“峰值IgG”。在研究的两个个体中，早在加强免疫后2周就可以检测到自身抗- id IgG，并且在整个研究期间都可以检测到。通过研究a)系列IgG样品抑制125I-IgG F（ab’）2抗TT与兔抗id抗体结合的能力，以及b)兔抗id特异性抑制125I-TT与血清IgG系列样品结合的能力，获得了TT加强免疫后IgG F（ab’）2抗TT的独特型表达调节的证据。两项试验都表明，在加强免疫之前和之后不久获得的抗tt上存在的一些独特型决定因子在免疫后2周及以后的表达程度要小得多。这些决定因子表达的减少与自身抗- id抗体的出现一致，并且在整个4个月的研究期间保持明显。所获得的结果表明，自体抗- id抗体在正常人类受试者经过抗原二次攻击后产生，并提示这些抗体可能在抗原加强免疫后观察到的独特型表达调节中起作用。
Auto-anti-idiotypic antibodies were searched for in the serum of two individuals who received booster immunization with tetanus toxoid (TT) antigen. IgG from serial bleeds obtained for 4 mo after immunization was studied a) for its capacity to specifically bind 125I IgG F(ab')2 anti-TT prepared from serum obtained 7 and 10 days post-booster immunization, and b) for its capacity (after its absorption with TT) to specifically inhibit the binding of 125I-radiolabeled TT to IgG obtained 7 and 10 days post-immunization and designated "peak IgG." In both individuals studied, auto-anti-Id IgG were detected in both assays as early as 2 wk post-booster immunization and remained detectable throughout the study period. Evidence for modulation of idiotypic expression on IgG F(ab')2 anti-TT after booster immunization with TT was obtained by studying a) the capacity of serial IgG samples to inhibit the binding of 125I-IgG F(ab')2 anti-TT to rabbit anti-Id antibody raised against the IgG F(ab')2 anti-TT, and b) the capacity of the rabbit anti-Id to specifically inhibit the binding of 125I-TT to serial samples of serum IgG. Both assays indicated that some idiotypic determinants present on anti-TT obtained before and shortly after booster immunization were expressed to a much lesser extent 2 wk and beyond after immunization. The decrease in the expression of these determinants coincided with the appearance of auto-anti-Id antibody and remained evident throughout the 4-mo study period. The results obtained indicate that auto-anti-Id antibodies arise in normal human subjects after a secondary challenge with antigen and suggest that these antibodies may play a role in the modulation of idiotypic expression that is observed after booster immunization with antigen.