A putative Plasmodium falciparum exported serine/threonine protein kinase
A putative Plasmodium falciparum exported serine/threonine protein kinase
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DOI：
10.1016/s0166-6851(96)02805-8
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发表时间：
1997-03-01
影响因子：
1.5
通讯作者：
Anders, RF
中科院分区：
文献类型：
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作者：
Kun, JFJ;Hibbs, AR;Anders, RF
恶性疟原虫丝氨酸/苏氨酸蛋白激酶的8kb基因已被克隆和测序。该基因由两个外显子组成：外显子I为2kb，外显子II为5.6kb。该基因编码2510个氨基酸的大蛋白质。针对融合蛋白产生的抗体被用来定位假定的激酶。免疫荧光显微镜下，感染红细胞胞浆内可见。通过免疫电子显微镜观察，它与红细胞膜结构和红细胞膜有关，特别是在寄生虫诱导的结节上。这是第一个推测的恶性疟原虫蛋白激酶从寄生虫输出到它的宿主细胞。(C)1997年爱思唯尔科学公司。
An 8kb gene coding for a putative serine/threonine protein kinase from Plasmodium falciparum has been cloned and sequenced. It is arranged in two exons: exon I is 2 kb and exon II is 5.6 kb. The gene codes for a large protein of 2510 amino acids. Antibodies raised against a fusion protein were used to localize the putative kinase. By immunofluorescence microscopy, it was found in the cytoplasm of infected red cells. By immunoelectron microscopy it was associated with membranous structures in the red cell and with the red cell membrane, particularly at parasite-induced knobs. This is the first putative protein kinase of P. falciparium to be exported from the parasite into its host cell. (C) 1997 Elsevier Science B.V.