L‐arginine induces dopamine release from the striatum in vivo
L‐arginine induces dopamine release from the striatum in vivo
复制标题
L-精氨酸诱导体内纹状体释放多巴胺
作者：
A. Strasser;R. McCarron;H. Ishii;D. Stanimirovic;M. Spatz
最近的报道表明，诱导一氧化氮（NO）引起多巴胺（DA）从纹状体在体外释放。在这项研究中，我们使用L-精氨酸（L-Arg）证明在体内刺激蒙古沙土鼠纹状体DA释放的微透析。与对照组相比，在灌流液中存在L-Arg的情况下，纹状体细胞外液（ECF）中的DA含量增加7-15倍（无药物灌流液中的DA水平为0.050 ± 0.009至0.092 ± 0.023 pmol 10 μUl−1）。同时灌注L-Arg和一氧化氮合酶抑制剂硝基-L-精氨酸（NLA），显著降低L-Arg对纹状体DA释放的影响。NLA灌注的动物含有DA水平显着低于对照纹状体透析液中观察到的。这些研究结果表明，第一次在体内DA的释放可以诱导L-精氨酸，NO的前体。数据强烈表明，NO可能调节纹状体DA的释放。
Recent reports indicate that induction of nitric oxide (NO) evokes dopamine (DA) release from the striatum in vitro. In this study, we used L-arginine (L-Arg) to demonstrate the in vivo stimulation of DA release from the striatum of Mongolian gerbils using microdialysis. The content of DA in the striatal extracellular fluid (ECF) increased 7–15-fold in the presence of L-Arg in the perfusate as compared with that of the controls (DA level in drug-free perfusate varied from 0.050 ± 0.009 to 0.092 ± 0.023 pmol 10 μUl−1). Simultaneous perfusion of L-Arg with nitro-L-arginine (NLA), an inhibitor of nitric oxide synthase, markedly reduced the L-Arg effect on DA release from the striatum. The NLA-perfused animals contained DA levels significantly lower than those observed in the control striatal dialysate. These findings indicate for the first time that DA release in vivo can be induced by L-Arg, the precursor of NO. The data strongly suggest that NO may modulate striatal DA release.