Differential expression of WNTs and FRPs in the synovium of rheumatoid arthritis and osteoarthritis
Differential expression of WNTs and FRPs in the synovium of rheumatoid arthritis and osteoarthritis
复制标题
DOI：
10.1016/j.bbrc.2006.05.075
复制
发表时间：
2006-07-14
影响因子：
3.1
通讯作者：
Okada, Yasunori
中科院分区：
文献类型：
--
作者：
Imai, Kazushi;Morikawa, Masako;Okada, Yasunori
关节滑膜细胞在类风湿性关节炎(RA)的进展中起着关键作用。然而，触发RA滑膜细胞侵袭的机制(S)尚不清楚，而不是其他关节疾病，如骨关节炎(OA)。在这里，我们用逆转录-聚合酶链式反应检测了WNT及其抑制物--分泌卷曲相关蛋白(FRP)在RA和OA滑膜中的表达。WNT10B在RA滑膜中表达最多，FRP1、FRP2和FRP4在OA滑膜中表达最多。免疫组织化学显示WNT10B和FRP1分别定位于RA和OA滑膜衬里细胞、成纤维细胞和内皮细胞，且WNT10B的表达随炎性细胞浸润和组织纤维化程度的加重而增加。WNT10B和WNT信号通路可上调膜型基质金属蛋白(MT1MMPs)的表达。MT1MMP免疫定位于与WNT10B和P-catenin染色相同的细胞。本研究表明，WNTs和FRPS在RA和OA滑膜中有不同的表达，提示它们参与了这些疾病的病理过程。(C)2006 Elsevier Inc.保留所有权利。
Synovial cells of the joint play a key role in the progression of rheumatoid arthritis (RA). However, the mechanism(s) that triggers aggression of RA synovial cells but not other arthropathies such as osteoarthritis (OA) is not clear. Here we examined expression of WNT and the WNT inhibitor, secreted frizzled-related protein (FRP), in RA and OA synovium by reverse transcription-PCR. WNT10B was most frequently detected in RA synovium, and FRP1, FRP2, and FRP4 in OA synovium. Immunohistochemistry localized WNT10B and FRP1 in synovial lining cells, fibroblasts, and endothelial cells in RA and OA synovium, respectively, and WNT10B expression was increased in parallel with the degree of inflammatory cell infiltration and tissue fibrosis. Membrane-type 1 matrix metalloprotemse (MT1MMP) was upregulated by WNT10B and activation of WNT signaling. MT1MMP immunolocalized to cells identical to WNT10B and P-catenin staining. The present study demonstrated that WNTs and FRPs are differentially expressed in RA and OA synovium, and suggests an involvement in the pathology of these diseases. (c) 2006 Elsevier Inc. All rights reserved.