Low-energy X-ray fluorescence microscopy opening new opportunities for bio-related research
Low-energy X-ray fluorescence microscopy opening new opportunities for bio-related research
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DOI：
10.1098/rsif.2009.0157.focus
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发表时间：
2009-10-06
影响因子：
3.9
通讯作者：
Kiskinova, Maya
中科院分区：
文献类型：
--
作者：
Kaulich, Burkhard;Gianoncelli, Alessandra;Kiskinova, Maya
生物系统是独特的物质，具有非常复杂的形态和以轻元素为主的高度异质的化学成分。在亚微米水平上定性区分组成元素的横向分布，并将其与亚细胞生物结构相关联，仍然是一个挑战。最近在 TwinMic 扫描传输模式下实施的低能 X 射线荧光显微光谱技术，为绘制轻元素分布图开辟了新的机会，并辅以同时采集吸收和相衬图像所提供的形态信息。通过对海洋生物学和食品科学感兴趣的样本进行的两个试点实验，证明了可以在生物相关研究领域获得的重要新信息。他们证明了对 Tintinnopsis radix lorica 中必需微量元素的结构和组成富集、分布和相关性以及小麦种子中微量营养素的横向分布产生重要见解的潜力。
Biological systems are unique matter with very complex morphology and highly heterogeneous chemical composition dominated by light elements. Discriminating qualitatively at the sub-micrometer level the lateral distribution of constituent elements, and correlating it to the sub-cellular biological structure, continues to be a challenge. The low-energy X-ray fluorescence microspectroscopy, recently implemented in TwinMic scanning transmission mode, has opened up new opportunities for mapping the distribution of the light elements, complemented by morphology information provided by simultaneous acquisition of absorption and phase contrast images. The important new information that can be obtained in bio-related research domains is demonstrated by two pilot experiments with specimens of interest for marine biology and food science. They demonstrate the potential to yield important insights into the structural and compositional enrichment, distribution and correlation of essential trace elements in the lorica of Tintinnopsis radix, and the lateral distribution of trace nutrients in the seeds of wheat Triticum aestivum.