Tea prepared from Anastatica hirerochuntica seeds contains a diversity of antioxidant flavonoids, chlorogenic acids and phenolic compounds

Tea prepared from Anastatica hirerochuntica seeds contains a diversity of antioxidant flavonoids, chlorogenic acids and phenolic compounds
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DOI:
10.1016/j.phytochem.2010.11.017
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发表时间:
2011-02-01
期刊:
影响因子:
3.8
通讯作者:
Crozier, Alan
Crozier, Alan
中科院分区:
生物学2区
文献类型:
--
作者:
AlGamdi, Noura;Mullen, William;Crozier, Alan

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HPLC-PDA-MS 2用于鉴定由Anastatica hireochuntica种子制成的草药茶中的酚类和多酚类化合物,Anastatica hireochuntica是一种在撒哈拉-阿拉伯沙漠中发现的植物,用于治疗各种疾病。20个化合物,包括一系列的黄酮C-和O-连接的糖苷,酚酸,绿原酸和黄酮醇进行了鉴定或部分鉴定的基础上,与参考化合物和MS 2和MS 3碎片模式的共色谱。黄酮类化合物为主要成分,以木犀草素、芹菜素和薯蓣皂苷结合物的形式存在。使用HPLC和在线ABTS(中心点+)检测系统评估Anastatica中单个化合物的抗氧化潜力。结果表明,14个化合物具有抗氧化活性。茶的抗氧化能力的最高贡献,56%,来自3,4-二羟基苯甲酸和咖啡酰-和二咖啡酰奎宁酸,而6-C-葡萄糖苷的木犀草素和芹菜素贡献41%。(C)2010爱思唯尔有限公司保留所有权利。
HPLC-PDA-MS2 was used to identify phenolic and polyphenolic compounds in an herbal tea made from seeds of Anastatica hirerochuntica, a plant found in the Sahara-Arabian deserts and used to treat a variety of ailments. Twenty compounds comprising a series of flavone C- and O-linked glycosides, phenolic acids, chlorogenic acids and flavonols were identified or partially identified on the basis of co-chromatography with reference compounds and MS2 and MS3 fragmentation patterns. The flavones were the principal components, occurring as luteolin, apigenin and diosmetin conjugates. The antioxidant potential of individual compounds in Anastatica was assessed using HPLC with an on-line ABTS(center dot+) detection system. This revealed that 14 compounds exhibited antioxidant activity. The highest contribution to the antioxidant capacity of the tea, 56%, came from 3,4-dihydroxybenzoic acid and caffeoyl- and dicaffeoylquinic acids while 6-C-glucosides of luteolin and apigenin contributed 41%. (C) 2010 Elsevier Ltd. All rights reserved.