Rapid Intracellular Growth of Gold Nanostructures Assisted by Functionalized Graphene Oxide and Its Application for Surface-Enhanced Raman Spectroscopy
Rapid Intracellular Growth of Gold Nanostructures Assisted by Functionalized Graphene Oxide and Its Application for Surface-Enhanced Raman Spectroscopy
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功能化氧化石墨烯辅助金纳米结构的快速细胞内生长及其在表面增强拉曼光谱中的应用
DOI：
10.1021/ac3023907
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发表时间：
2012-12-04
影响因子：
7.4
通讯作者：
Guo, Zhouyi
中科院分区：
文献类型：
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作者：
Liu, Zhiming;Hu, Chaofan;Guo, Zhouyi
近年来，金属纳米粒子与氧化石墨烯杂化用于高性能表面增强拉曼散射（SERS）引起了极大的关注。在此，我们提出了一种在聚乙烯吡咯烷酮（PVP）功能化氧化石墨烯（GO）辅助下在细胞内一锅法绿色合成金纳米结构的方法。所获得的杂化物[GO/PVP/细胞内生长的金纳米粒子（IGAuNs）]随机分散在整个细胞中。与IGAuNs相比，GO/PVP/IGAuNs的生长显著加快，这可归因于富集在GO上的PVP的配位作用。GO/PVP/IGAuNs可作为优异的SERS探针，用于超灵敏检测位于细胞质、核质和核仁中的癌细胞成分。GO/PVP/IGAuNs在细胞内的随机分布有利于对细胞成分进行有效的拉曼表征，拉曼图像中SERS信号的均匀分布证实了这一点。由GO/PVP/IGAuNs诱导的SERS信号最早可在15小时被收集，这使得能够快速检测肿瘤细胞。总之，这种简便的、用于GO/PVP/IGAuNs在细胞内快速生长的绿色策略为生物医学应用提供了巨大的潜力。
Hybridization of metal nanoparticles with graphene oxide for high performance surface-enhanced Raman scattering (SERS) has attracted overwhelming attention in recent years. Herein, a one-pot green route for intracellular synthesis of gold nanostructures assisted by poly(vinylpyrrolidone) (PVP)-functionalized graphene oxide (GO) was proposed. The hybrids obtained [GO/PVP/intracellularly grown gold nanoparticles (IGAuNs)] randomly scattered throughout the cell. Compared with the IGAuNs, the growth of GO/PVP/IGAuNs was remarkably accelerated, which could be attributed to the coordination of PVP enriched on GO. GO/PVP/IGAuNs could serve as excellent SERS probes for ultrasensitive detection of cellular components of cancer cells located in the cytoplasm, nucleoplasm, and nucleolus. The random intracellular distribution of GO/PVP/IGAuNs facilitated the effective Raman characterization of cellular components, which was confirmed by the uniform distribution of SERS signals in the Raman image. The SEAS signals induced by GO/PVP/IGAuNs could be collected as early as 15 h, which allowed rapid detection of tumor cells. In conclusion, this facile and green strategy for fast intracellular growth of GO/PVP/IGAuNs offered great potential for biomedical applications.