FUNCTIONAL-PROPERTIES OF NONHUMAN PRIMATE ANTIBODY TO PORPHYROMONAS-GINGIVALIS
FUNCTIONAL-PROPERTIES OF NONHUMAN PRIMATE ANTIBODY TO PORPHYROMONAS-GINGIVALIS
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DOI：
10.1128/iai.63.9.3245-3252.1995
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发表时间：
1995-09-01
影响因子：
3.1
通讯作者：
NOVAK, MJ
中科院分区：
文献类型：
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作者：
ANDERSON, DM;EBERSOLE, JL;NOVAK, MJ
非人灵长类动物（NHP）是研究牙龈卟啉单胞菌相关牙周病中宿主-寄生虫相互作用的有用模型。本研究确定了NHP血清对(i)牙龈卟啉单胞菌的直接杀伤，（ii）牙龈卟啉诱导的超氧阴离子（O-2(-)）从人多形核白细胞（PMNs）释放的影响，以及（iii） PMNs结合和吞噬牙龈卟啉单胞菌的能力。使用了三种类型的NHP血清：(i)正常或基线血清；结扎性牙周炎后获得的血清；（iii）正规化牙龈卟啉卟啉菌主动免疫后获得的血清。所有测定均在添加或不添加人补体的情况下进行。免疫血清和补体对牙龈假单胞菌的直接杀伤能力显著高于其他处理（P < 0.01），结扎性牙周炎NHPs血清的杀伤能力显著低于含有牙龈假单胞菌定植天然抗体的基线血清（P < 0.03）。免疫后的NHPs血清可诱导牙龈卟噬菌释放O-2（-）水平显著升高（P < 0.01）。最后，免疫NHPs的血清显著增强了PMNs对牙龈卟啉卟啉的摄取（P < 0.009），尽管三种血清类型的细菌对PMNs的结合相似，但用牙龈卟啉卟啉主动免疫NHPs可诱导功能抗体增强直接杀伤，积极影响PMNs的活化，增强PMNs对牙龈卟啉卟啉的吞噬能力。此外，作为进展性牙周炎后遗症产生的抗体似乎缺乏这些功能。研究人员指出，个体NHPs血清的功能能力存在很大差异，这可能导致个体对牙龈卟啉菌引起的疾病的易感性。这种可变性表明，血清抗体功能试验的结果可能有助于预测宿主对疾病的易感性和对治疗的反应。
The nonhuman primate (NHP) serves as a useful model for examining the host-parasite interactions in Porphyromonas gingivalis-associated periodontal disease. This study determined the influence of NHP sera on (i) the direct killing of P. gingivalis, (ii) P, gingivalis-induced superoxide anion (O-2(-)) release from human polymorphonuclear leukocytes (PMNs), and (iii) the ability of PMNs to bind and phagocytize P. gingivalis. Three types of NHP sera were utilized: (i) normal or baseline sera; (ii) sera obtained after ligature-induced periodontitis; and (iii) sera obtained following active immunization with formalinized P. gingivalis. All assays were performed with or without the addition of human complement. Significantly more (P < 0.01) direct killing of P. gingivalis occurred with immunized sera and complement than with any of the other treatments, The sera from ligature-induced periodontitis NHPs had significantly less (P < 0.03) killing capacity than the baseline sera, which contained natural antibody produced to P. gingivalis colonization. Sera from immunized NHPs were used to opsonize P. gingivalis and caused significantly greater (P < 0.01) levels of O-2(-) release from PMNs. Finally, the sera from immunized NHPs significantly enhanced (P < 0.009) the uptake of P. gingivalis by PMNs, although binding of the bacteria to PMNs was similar among all three serum types, Active immunization of NHPs with P. gingivalis elicited a functional antibody that enhanced direct killing, positively influenced the activation of PMNs, and enhanced the ability of PMNs to phagocytize P. gingivalis. Moreover, antibody produced as a sequela of progressing periodontitis appeared to lack these functions. A wide variability in functional capacity of the sera from individual NHPs, which may contribute to an individual's susceptibility to P. gingivalis-induced disease, aas noted. This variability suggested that results from functional tests of serum antibody may aid in predicting host susceptibility to disease and response to therapy.