Female and male microglia are not different in the dentate gyrus of postnatal day 10 mice.
Female and male microglia are not different in the dentate gyrus of postnatal day 10 mice.
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出生后第 10 天小鼠的齿状回中雌性和雄性小胶质细胞没有差异。
DOI：
10.1016/j.neulet.2023.137171
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发表时间：
2023
影响因子：
2.5
通讯作者：
Eisch,AmeliaJ
中科院分区：
文献类型：
--
作者：
Guez-Barber,Danielle;Colon,LoriannaM;Raphael,Dana;Wragan,MaxA;Yun,Sanghee;Eisch,AmeliaJ
小胶质细胞，大脑的常驻免疫细胞，支持正常的大脑功能和大脑对疾病和损伤的反应。海马齿状回（DG）是研究小胶质细胞的重要部位，因为它在许多行为和认知功能中起着重要作用。有趣的是，小胶质细胞和相关细胞在雌性与雄性啮齿动物中是不同的，即使在生命早期。事实上，出生后天（P）依赖性的小胶质细胞的数量，密度和形态的差异已被报告在特定年龄的某些海马亚区。然而，尚未在P10评估DG的性别差异，P10是一个与人类足月妊娠的啮齿动物神经解剖学等效的诊断相关时间点。为了解决这一知识差距，分析了雌性和雄性C57 BL/6 J小鼠DG中的Iba 1+细胞（在门和分子层中富集）的数量（通过体视学）和密度（通过体视学和通过采样）。接下来，将Iba 1+细胞分类到先前在文献中建立的形态学类别中。最后，将每个形态学类别中Iba 1+细胞的百分比乘以总细胞数，以生成每个类别中Iba 1+细胞的总数。结果显示，在P10门或分子层中，Iba 1+细胞数量、密度或形态无性别差异。使用常用的方法（采样、体视学、形态学分类），P10 DG中Iba 1+细胞缺乏性别差异，这为解释损伤后观察到的小胶质细胞变化提供了基线。
Microglia, the resident immune cells of the brain, support normal brain function and the brain’s response to disease and injury. The hippocampal dentate gyrus (DG) is important for microglial study due to its central role in many behavioral and cognitive functions. Interestingly, microglia and related cells are distinct in female vs. male rodents, even in early life. Indeed, postnatal day (P)-dependent sex differences in number, density, and morphology of microglia have been reported in certain hippocampal subregions at specific ages. However, sex differences in the DG have not yet been assessed at P10, a translationally relevant time point as the rodent neuroanatomical eqivalent of human term gestation. To address this knowledge gap, Iba1+ cells in the DG (which are enriched in the Hilus and Molecular Layer) in female and male C57BL/6J mice were analyzed for their number (via stereology) and density (via stereology and via sampling). Next, Iba1+ cells were classified into morphology categories previously established in the literature. Finally, the percent of Iba1+ cells in each morphology category was multiplied by total cell number to generate a total number of Iba1+ cells in each category. Results show no sex difference in Iba1+ cell number, density, or morphology in the P10 Hilus or Molecular Layer. The lack of sex difference in Iba1+ cells in P10 DG using commonly-employed methodologies (sampling, stereology, morphology classification) provides a baseline from which to interpret microglia changes seen after injury.