The production of protein-loaded microparticles by supercritical fluid enhanced mixing and spraying
The production of protein-loaded microparticles by supercritical fluid enhanced mixing and spraying
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DOI：
10.1016/j.jconrel.2004.07.017
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发表时间：
2005-01-03
影响因子：
10.8
通讯作者：
Shakesheff, KM
中科院分区：
文献类型：
--
作者：
Whitaker, MJ;Hao, JY;Shakesheff, KM
在这项研究中，我们使用超临界二氧化碳作为加工介质的聚（DL-乳酸）P（（DL）LA）微粒，封装蛋白质材料的制造。我们之前已经证明，这种聚合物和蛋白质的干粉可以在超临界二氧化碳条件下（高于31.1摄氏度和73.8巴）混合，并且蛋白质组分保持其生物活性。在本文中，我们的工作进展表明，塑化聚合物和干粉蛋白质混合物可以喷雾形成固体聚合物颗粒，封装蛋白质。喷涂后的粒度范围为10 - 300 μ m。核糖核酸酶A和溶菌酶被封装在聚合物中，酶活性没有显著损失。胰岛素和降钙素的生物测定证实了在微粒制造和肽/蛋白质释放后活性的保留。(c)2004 Elsevier B. V.保留所有权利。
In this study, we use supercritical carbon dioxide as a processing medium for the fabrication of poly(DL-lactic acid) P((DL)LA) microparticles that encapsulate a protein material. We have previously demonstrated that this polymer and a dry powder of a protein can be mixed under supercritical carbon dioxide conditions (above 31.1 degrees C and 73.8 bar) and that the protein component retains its biological activity. In this paper, we progress the work to demonstrate that the plasticized polymer and dry powder protein mixture can be sprayed to form solid polymer particles that encapsulate the protein. Particle size range is between 10 and 300 mu m after spraying. Ribonuclease A and lysozyme were encapsulated in the polymer without significant loss of enzymatic activity. Biological assays of insulin and calcitonin confirm retention of activity after fabrication of the microparticles and release of the peptides/proteins. (c) 2004 Elsevier B.V. All rights reserved.