A plant-transformation-competent BIBAC/BAC-based map of rice for functional analysis and genetic engineering of its genomic sequence
A plant-transformation-competent BIBAC/BAC-based map of rice for functional analysis and genetic engineering of its genomic sequence
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DOI：
10.1139/g07-006
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发表时间：
2007-03-01
期刊：
影响因子：
3.1
通讯作者：
Zhang, Hong-Bin
中科院分区：
文献类型：
--
作者：
Li, Yaning;Uhm, Taesik;Zhang, Hong-Bin
水稻基因组测序为水稻和其他谷类物种的功能基因组学研究提供了平台。然而，需要多种方法来确定其基因和序列的功能，并利用基因组测序结果进行谷类作物的遗传改良。在这里，我们报告了一种具有植物转化能力的、基于二元细菌人工染色体（BIBAC）和细菌人工染色体（BAC）的水稻图谱，以促进这些研究。该图谱由 20 835 个 BIBAC 和 BAC 克隆构建，由 579 个重叠的 BIBAC/BAC 重叠群组成。为了便于对 8 号染色体基因组序列进行功能分析，并克隆映射到该染色体的基因和 QTL，我们将染色体重叠群锚定到现有的水稻遗传图谱上。染色体图谱由 I1 重叠群、59 个遗传标记和 36 个序列标记位点组成，总共跨越约 1 个重叠群。物理长度为 38 Mb。 8号染色体遗传图谱与物理图谱的对比分析表明，除着丝粒区的“冷点”外，沿染色体臂还存在3个基因重组“热点”和2个“冷点”，提示染色体的序列成分含量可能影响其局部基因重组频率。由于其植物可转化性，BIBAC/BAC图谱可以为水稻基因组序列的功能分析以及有效利用测序结果进行基因和QTL克隆和分子育种提供平台。
Sequencing of the rice genome has provided a platform for functional genomics research of rice and other cereal species. However, multiple approaches are needed to determine the functions of its genes and sequences and to use the genome sequencing results for genetic improvement of cereal crops. Here, we report a plant-transformation-competent, binary bacterial artificial chromosome (BIBAC) and bacterial artificial chromosome (BAC) based map of rice to facilitate these studies. The map was constructed from 20 835 BIBAC and BAC clones, and consisted of 579 overlapping BIBAC/ BAC contigs. To facilitate functional analysis of chromosome 8 genomic sequence and cloning of the genes and QTLs mapped to the chromosome, we anchored the chromosomal contigs to the existing rice genetic maps. The chromosomal map consists of I I contigs, 59 genetic markers, and 36 sequence tagged sites, spanning a total of ca. 38 Mb in physical length. Comparative analysis between the genetic and physical maps of chromosome 8 showed that there are 3 "hot" and 2 "cold" spots of genetic recombination along the chromosomal arms in addition to the "cold spot" in the centromeric region, suggesting that the sequence component contents of a chromosome may affect its local genetic recombination frequencies. Because of its plant transformability, the BIBAC/BAC map could provide a platform for functional analysis of the rice genome sequence and effective use of the sequencing results for gene and QTL cloning and molecular breeding.