Assisted reproductive technologies impair the expression and methylation of insulin-induced gene 1 and sterol regulatory element-binding factor 1 in the fetus and placenta
Assisted reproductive technologies impair the expression and methylation of insulin-induced gene 1 and sterol regulatory element-binding factor 1 in the fetus and placenta
复制标题
辅助生殖技术损害胎儿和胎盘中胰岛素诱导基因1和甾醇调节元件结合因子1的表达和甲基化
DOI：
10.1016/j.fertnstert.2013.12.034
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发表时间：
2014-04-01
影响因子：
6.7
通讯作者：
Jin, Fan
中科院分区：
文献类型：
--
作者：
Lou, Hangying;Le, Fang;Jin, Fan
目的：通过分析胎儿和胎盘中胰岛素诱导基因（INSIG）、固醇调节元件结合蛋白（SREBP）和SREBP裂解激活蛋白的表达水平和DNA甲基化模式，评估与辅助生殖技术（ART）相关的胆固醇代谢。设计：实验研究。设置：大学附属教学医院IVF中心。患者：招募了四组患者：IVF/卵胞浆内单精子注射（ICSI）后的妊娠（n = 55），自然妊娠（n = 40），IVF/ICSI后的多胎减少（n = 56），以及控制性卵巢过度刺激（COH）后的多胎减少（n = 42）。干预：测定胎儿和胎盘样品中INSIG-SREBP-SREBP切割激活蛋白的表达和DNA甲基化。结果：ICSI治疗组脐血中甘油三酯和载脂蛋白B水平显著高于对照组（P < 0. 05），ICSI治疗组脐血中甘油三酯和载脂蛋白B水平显著高于对照组（P < 0. 05）。同时，在ICSI妊娠的胎儿中，INSIG 1的表达显著高于IVF组和对照组，甲基化率低于IVF组和对照组。此外，在胎盘中，INSIG 1和SREBF 1的转录也显着较高的甲基化率较低的ICSI组比IVF和control groups. Conclusions（s）：我们的研究结果表明，INSIG 1和SREBF 1的失调引起的ART不仅在胎儿，而且在胎盘中，主要是在ICSI组。然而，这种失调的长期后遗症应密切关注。(c)2014年，美国生殖医学会（American Society for Reproductive Medicine）
Objective: To evaluate the cholesterol metabolism linked to assisted reproductive technology (ART) by analyzing the expression levels and DNA methylation patterns of the insulin-induced gene (INSIG), sterol regulatory element-binding protein (SREBP), and SREBP cleavage-activating protein in the fetus and placenta.Design: Experimental research study.Setting: An IVF center, university-affiliated teaching hospital.Patient(s): Four patients groups were recruited: pregnancies after IVF/intracytoplasmic sperm injection (ICSI) (n = 55), natural pregnancies (n = 40), multifetal reduction after IVF/ICSI (n = 56), andmultifetal reduction after controlled ovarian hyperstimulation (COH) (n-42).Intervention(s): Expression and DNA methylation of INSIG-SREBP-SREBP cleavage-activating protein in the fetus and placenta samples were determined.Main Outcome Measure(s): The expression and DNA methylation patterns were tested by real-time quantitative polymerase chain reaction (PCR) and pyrosequencing.Result(s): In the ICSI treatment group, significantly higher levels of triglycerides and apolipoprotein-B were observed in cord blood compared with controls. Meanwhile, in ICSI-conceived fetuses, the expression of INSIG1 was significantly higher, and methylation rates were lower, than in the IVF and control groups. Furthermore, in the placenta, the INSIG1 and SREBF1 transcripts were also significantly higher with lower methylation rates in the ICSI group than in the IVF and control groups.Conclusion(s): Our results indicated that the dysregulation of INSIG1 and SREBF1 caused by ART were observed not only in the fetus but also in the placenta, primarily in the ICSI group. However, the long-term sequelae of this dysregulation should be closely followed. (c) 2014 by American Society for Reproductive Medicine.