Biological similarities of rat fibroblast interferon to human and mouse alpha interferons.
Biological similarities of rat fibroblast interferon to human and mouse alpha interferons.
复制标题
大鼠成纤维细胞干扰素与人和小鼠α干扰素的生物学相似性。
DOI：
10.1099/0022-1317-66-9-1961
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
Murasko,DM
中科院分区：
文献类型：
--
作者：
Davis,CT;Rusckowski,M;Murasko,DM
用纽卡斯尔病毒处理大鼠胚胎成纤维细胞（CD细胞），可产生高滴度的大鼠干扰素（IFN）。经SDS-PAGE电泳鉴定，在30 K ~ 33 K、25 K ~ 27 K和17 K ~ 22 K mol.重量抗体亲和层析显示，95%的CD IFN与抗人白细胞IFN抗体柱结合，34%与抗小鼠L细胞IFN抗体柱结合，没有与抗人成纤维细胞IFN抗体柱结合。与抗人白细胞IFN抗体柱结合的IFN含有所有三种分子量的IFN。然而，与抗小鼠L细胞IFN抗体柱结合的材料仅显示30 K和20 K物种的IFN活性。未分离的CD IFN和通过抗体亲和层析分离的IFN的异源抗病毒活性的检查揭示了相同的活性模式：对豚鼠细胞具有35 - 50%的同源活性，对小鼠细胞具有10 - 20%的同源活性，对人细胞具有2 - 4%的同源活性，对牛细胞没有同源活性。这些数据表明，这种大鼠成纤维细胞IFN的主要部分与其他物种产生的α IFN有关。
High titres of rat interferon (IFN) can be produced by rat embryo fibroblast (CD) cells after treatment with Newcastle disease virus. This CD IFN was characterized by SDS–PAGE, and was found to contain three species at 30K to 33K, 25K to 27K and 17K to 22K mol. wt. Antibody affinity chromatography revealed that 95% of the CD IFN bound to an anti-human leukocyte IFN antibody column, 34% to an anti-mouse L cell IFN antibody column and none to an anti-human fibroblast IFN antibody column. The IFN that bound to the anti-human leukocyte IFN antibody column contained all three molecular weight species of IFN. However, the material bound to the anti-mouse L cell IFN antibody column only showed IFN activity of the 30K and 20K species. Examination of the heterologous antiviral activity of the unseparated CD IFN and of the IFN separated by antibody affinity chromatography revealed the same patterns of activity: 35 to 50% of homologous activity on guinea-pig cells, 10 to 20% on mouse cells, 2 to 4% on human cells and none on bovine cells. The data suggest that a major portion of this rat fibroblast IFN is related to alpha IFNs produced by other species.