MODULATION OF SELENIUM-DEPENDENT GLUTATHIONE-PEROXIDASE BY PERFLUORODECANOIC ACID IN RATS - EFFECT OF DIETARY SELENIUM
MODULATION OF SELENIUM-DEPENDENT GLUTATHIONE-PEROXIDASE BY PERFLUORODECANOIC ACID IN RATS - EFFECT OF DIETARY SELENIUM
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DOI：
10.1093/jn/120.3.298
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发表时间：
1990-03-01
影响因子：
4.2
通讯作者：
ROBERTSON, LW
中科院分区：
文献类型：
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作者：
CHEN, LC;BORGES, T;ROBERTSON, LW
48只雄性Sprague-Dawley大鼠，按0.4、0.2或1.0mg/kg饲料硒（Se）的剂量，一次性注射全氟癸酸（PFDA）玉米油溶液，2 wk后处死。对照组动物成对喂食，并用等体积的溶剂处理。PFDA治疗显着增加硒依赖性谷胱甘肽过氧化物酶（Se-GSHPx）的活性在大鼠肝细胞质中喂养的0.04毫克硒/公斤的饮食，但在大鼠喂养的其他饮食。0.04mg/kg硒组大鼠肝细胞内Se-GSHPx活性升高，血清硒含量和Se-GSHPx活性升高。用酶联免疫吸附法测定硒-谷胱甘肽过氧化物酶（Se-GSHPx），结果表明，PFDA可引起0.2或1.0mg硒/kg饲料喂养大鼠的Se-GSHPx蛋白降低，但对0.04mg硒/kg饲料喂养的大鼠无影响。进一步分析表明，在所有三个组中，PFDA均增加Se-GSHPx活性与抗体反应蛋白的比值。体外直接将PFDA加入Se-GSHPx活性测定混合物中未产生任何影响。在所有三组中，PFDA治疗显著增加还原型谷胱甘肽。结果表明，PFDA对大鼠肝硒含量、Se-GSHPx活性和Se-GSHPx蛋白均有影响，且这种影响与膳食硒水平和肝硒水平有关。
Forty-eight male Sprague-Dawley rats fed a diet containing 0.4, 0.2 or 1.0 mg of selenium (Se)/kg of diet were injected with a single dose (35 mg/kg) of perfluorodecanoic acid (PFDA) in corn oil and killed 2 wk later. Control animals were pair-fed and treated with an equal volume of vehicle. PFDA treatment significantly increased Se-dependent glutathione peroxidase (Se-GSHPx) activity in liver cytosol of rats fed the 0.04 mg of Se/kg of diet but not in rats fed the other diets. The increase in liver cytosolic Se-GSHPx activity in rats fed 0.04 mg of Se/kg of diet paralleled increases in Se content and serum Se-GSHPx activity. Determination of Se-GSHPx by an enzyme-linked immunosorbent assay showed that PFDA caused a decrease in Se-GSHPx protein in rats fed 0.2 or 1.0 mg of Se-kg of diet but not in rats fed 0.04 mg of Se/kg of diet. Further analysis revealed that the ratio of Se-GSHPx activity to antibody-reactive protein was increased by PFDA in all three groups. The in vitro addition of PFDA directly to the assay mixture for Se-GSHPx activity did not produce any effect. Reduced, glutathione was significantly increased PFDA treatment in all three groups. These data show that PFDA affects the Se content, Se-GSHPx activity and Se-GSHPx protein in rat liver and that the effect is dependent on the dietary/hepatic Se level.