Characterization and expression analysis of tandemly-replicated asc genes in the Japanese medaka, Oryzias latipes
Characterization and expression analysis of tandemly-replicated asc genes in the Japanese medaka, Oryzias latipes
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DOI：
10.1016/j.dci.2020.103894
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发表时间：
2021-02-01
影响因子：
2.9
通讯作者：
Hikima, Jun-ichi
中科院分区：
文献类型：
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作者：
Morimoto, Natsuki;Okamura, Yo;Hikima, Jun-ichi
ASC是炎症体的组成部分，在炎症反应中起着至关重要的作用。在哺乳动物中，ASC诱导下垂和炎性细胞因子的产生。在本研究中，我们鉴定并鉴定了青冈的3个AsC基因(asc1、asc2和asc3)。这些ASC基因是16号染色体上的串联复制，它们之间的外显子-内含子结构不同。这三种ASCs都保守吡喃和半胱氨酸天冬氨酸氨基转移酶募集结构域，这两个结构域对炎症体的形成很重要。在系统发育分析中，所有的ASCs都与其他硬骨鱼的ASCs聚在一起。ASC1在几个器官中的表达水平显著高于ASC2和ASC3，这表明ASC1可能是青竹的优势ASC。这三种ASC基因在嗜水气单胞菌和鱼爱德华氏菌感染过程中的表达模式不同。此外，Asc-1基因敲除的青冈细胞在ATP刺激12h后，Asc2的表达显著下调，而黑素刺激后Asc2的表达显著上调。此外，Asc-1基因敲除青竹的皮肤中Asc2和Asc3的表达显著高于野生型青冈。
ASC is a component of the inflammasome playing crucial roles in the inflammatory response. In mammals, ASC induces pyroptosis and inflammatory cytokine production. In this study, three asc genes (asc1, asc2, and asc3) from the Japanese medaka (Oryzias latipes) were identified and characterized. These asc genes were tandem replicates on chromosome 16, and their exon-intron structures differed between them. All three ASCs conserved the pyrin and caspase-recruitment domains, which are important for inflammasome formation. In phylogenetic analysis, all ASCs clustered with those of other teleosts. The asc1 expression levels were significantly higher in several organs than those of asc2 and asc3, suggesting that asc1 may act as a dominant asc in the Japanese medaka. Expression of the three asc genes showed different patterns during Aeromonas hydrophila and Edwardsiella piscicida infections. Furthermore, their expression was adequately down-regulated in the medaka fin derived cells stimulated with ATP for 12 h, while asc2 expression was statistically up-regulated after nigericin stimulation for 24 h. Moreover, the expression of asc2 and asc3 was significantly higher in the skin of ASC-1-knockout medaka than in that of the wild type medaka during A. hydrophila infection.