Transcripts of the nebulin gene from Ciona heart and their implications for the evolution of nebulin family genes.
Transcripts of the nebulin gene from Ciona heart and their implications for the evolution of nebulin family genes.
复制标题
海鞘心脏星云蛋白基因的转录本及其对星云蛋白家族基因进化的影响。
DOI：
10.1016/j.gene.2019.144036
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发表时间：
2019
期刊：
影响因子：
3.5
通讯作者：
Sumiko Kimura.
中科院分区：
文献类型：
--
作者：
Akira Nishika;Akira Hanashima;Satoshi Nakayama;Michio Ogasawara;Sumiko Kimura.
Nebulin 是一种 770kDa 的蛋白质，位于脊椎动物骨骼肌的细丝上。它也存在于文昌鱼的横纹肌中，文昌鱼是一种无脊椎动物头索动物，在系统发育上与脊椎动物接近。然而，尾索动物海鞘的星云蛋白或其在无脊椎动物心脏中的表达尚未得到研究。在这项研究中，我们研究了 Cionaintestinalis 中 nebulin 基因的结构和心脏表达，Cionaintestinalis 是一种尾索动物，其系统发育介于头索动物和脊椎动物之间。基因结构分析的结果，我们发现Cionanebulin基因预计为62kb，由143个外显子组成。星云蛋白预计由一个独特的 N 端区域、随后的 155 个星云蛋白重复序列、另一个独特区域、富含 Ser 的区域和 C 端 SH3 结构域组成。整体原位杂交实验表明Cionanebulin基因在多种肌肉中表达，包括心脏。然而，使用Cionanebulin抗体的蛋白质印迹分析没有检测到全长nebulin的存在。或者，对Cionaheart样品的RT-PCR实验检测到来自Cionanebulin基因的星云样和nrap样亚型的表达。这些结果表明，与脊椎动物心脏类似，Cionaharts 不表达 nebulin，而是表达 nrap 和 nebulette 样亚型。这些结果还表明，脊椎动物中的 nebulin、nebulette 和 nrap 基因在脊椎动物进化过程中与祖先无脊椎动物的 nebulin 基因分离。
Nebulin is a 770 kDa protein that is localized along the thin filaments of skeletal muscles in vertebrates. It is also present in the striated muscles of Amphioxus, an invertebrate cephalochordate that is phylogenetically close to vertebrates. However, the nebulin of urochordate ascidians or its expression in invertebrate hearts has not been investigated. In this study, we investigated the structure and cardiac expression of the nebulin gene in Cionaintestinalis, a urochordate whose phylogeny lies between cephalochordates and vertebrates. As a result of the gene structure analysis, we found that theCionanebulin gene predicted to be 62 kb and consists of 143 exons. The nebulin was expected to consist of a unique N-terminal region, followed by 155 nebulin repeats, another unique region, a Ser-rich region and a C-terminal SH3 domain. Whole-mountin situhybridization experiments showed that theCionanebulin gene was expressed in a variety of muscles, including hearts. However, Western blot analysis using antibody toCionanebulin did not detect the presence of full-length nebulin. Alternatively, RT-PCR experiments on samples ofCionaheart detected the expression of nebulette-like and nrap-like isoforms from theCionanebulin gene. These results indicate that, similarly to vertebrate hearts,Cionahearts do not express nebulin, but rather nrap- and nebulette-like isoforms. These results also imply that the nebulin, nebulette and nrap genes in vertebrates were separated from an ancestral invertebrate nebulin gene during vertebrate evolution.