Two novel isoneolacto-undecaglycosylceramides carrying Galα1→3Lewisx on the 6-linked antenna and N-acetylneuraminic acidα2→3 or galactose α1→3 on the 3-linked antenna, expressed in porcine kidney
Two novel isoneolacto-undecaglycosylceramides carrying Galα1→3Lewisx on the 6-linked antenna and N-acetylneuraminic acidα2→3 or galactose α1→3 on the 3-linked antenna, expressed in porcine kidney
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DOI：
10.1023/a:1006994126958
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发表时间：
1998-10-01
影响因子：
3
通讯作者：
Bouhours, JF
中科院分区：
文献类型：
--
作者：
Bouhours, D;Liaigre, J;Bouhours, JF
从猪肾皮质中分离出三种唾液酸化鞘糖脂和三种中性鞘糖脂，它们共享一个共同的异新乳糖核心。它们通过制备型 HPTLC 纯化，并通过部分外切糖苷酶水解、随后薄层色谱法和抗 Gal α 1-->3Gal、抗 2 型乳糖胺和抗 Lewis(x) 抗体的免疫染色、甲基化分析、MALDI-TOF 质谱和 H-1-NMR 光谱进行表征。中性糖脂中，一种是已知结构，(VIVI)-V-3'(3)(α Gal)(2)-iso-nLc(8)Cer，两种是因 Gal α 3Lewis(x) 决定簇数量不同而不同的新结构：(VIVI)-V-3'(3)(α Gal)、V'3 α Fuc-iso-nLc(8) 和(VIVI)-V-3'(3)(α Gal)(2)(VV)-V-3'(3)(α Fuc)(2)-iso-nLc(8)。在六联天线上发现了单个 Gala3Lewis(x) 行列式。在唾液酸化糖脂中，有两种先前已在其他物种和组织中发现，即 (VIVI)-V-3'(3)(NeuAc)(2)-iso-nLc(8) 和 VI(3)NeuAcVI'(3)alpha Gal-iso-nLc(8)。发现了一种新的结构，在 6 联天线上呈现 Gal α 3Lewis(x) 决定簇，在 6 联天线上呈现 N-乙酰神经氨酸，VI(3)NeuAcVI'(3 alpha)GalV'(3)alpha Fuc-iso-nLc(8)。这些结果表明，在体内，猪肾α 3岩藻糖基转移酶合成Gal α 3Lewis(x)决定簇，在3连接的Gal α 3新乳糖胺之前作用于6连接的Gal α 3新乳糖胺，并且似乎不能在新乳糖系列糖脂上合成唾液酸化的Lewis(x)决定簇。
Three sialosylated and three neutral glycosphingolipids sharing a common iso-neolacto core were isolated from porcine kidney cortex. They were purified by preparative HPTLC, and were characterized by partial exoglycosidase hydrolysis followed by thin layer chromatography and immunostaining with anti-Gal alpha 1-->3Gal, anti-type 2 lactosamine and anti-Lewis(x) antibodies, methylation analysis, MALDI-TOF mass spectrometry and H-1-NMR spectroscopy. Among neutral glycolipids, one was a known structure, (VIVI)-V-3'(3)(alpha Gal)(2)-iso-nLc(8)Cer, and two were novel structures differing by the number of Gal alpha 3Lewis(x) determinants: (VIVI)-V-3'(3)(alpha Gal),V'3 alpha Fuc-iso-nLc(8), and (VIVI)-V-3'(3)(alpha Gal)(2) (VV)-V-3'(3)(alpha Fuc)(2)-iso-nLc(8). The single Gala3Lewis(x) determinant was found on the 6-linked antenna. Among sialosylated glycolipids, two had been previously found in other species and tissues, (VIVI)-V-3'(3)(NeuAc)(2)-iso-nLc(8), and VI(3)NeuAcVI'(3)alpha Gal-iso-nLc(8). A novel structure was discovered presenting a Gal alpha 3Lewis(x) determinant on the 6-linked antenna and a N-acetylneuraminic acid on the 6-linked antenna, VI(3)NeuAcVI'(3 alpha)GalV'(3)alpha Fuc-iso-nLc(8). These results indicate that, in vivo, the porcine kidney alpha 3fucosyltransferase synthesizes the Gal alpha 3Lewis(x) determinant, acting on the 6-linked before the 3-linked Gal alpha 3neolactosamine, and appears unable to synthesize the sialosylated Lewis(x) determinant on neolactoseries glycolipids.