Telomeres in the mouse have large inter-chromosomal variations in the number of T(2)AG(3) repeats
Telomeres in the mouse have large inter-chromosomal variations in the number of T(2)AG(3) repeats
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DOI：
10.1073/pnas.94.14.7423
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发表时间：
1997-07-08
影响因子：
11.1
通讯作者：
Lansdorp, PM
中科院分区：
文献类型：
--
作者：
Zijlmans, JMJM;Martens, UM;Lansdorp, PM
已经在小鼠中观察到的超长端粒不符合关键端粒缩短与衰老和永生化相关的概念。在这里，我们已经使用定量荧光原位杂交来估计（T（2）AG（3））（n）长度的各个端粒在不同的小鼠品系。端粒长度是非常异质性的，但特定的染色体骨髓细胞和皮肤成纤维细胞从个别小鼠具有相似的端粒长度。我们估计最短的端粒长度约为10 kb，这表明每个小鼠细胞都有一些端粒的（T（2）AG（3））（n）长度在人类端粒的范围内。这些短的端粒可能是限制小鼠细胞复制潜力的关键。
The ultra-long telomeres that have been observed in mice are not in accordance with the concept that critical telomere shortening is related to aging and immortalization. Here, we have used quantitative fluorescence in situ hybridization to estimate (T(2)AG(3))(n) lengths of individual telomeres in various mouse strains. Telomere lengths were very heterogeneous, but specific chromosomes of bone marrow cells and skin fibroblasts from individual mice had similar telomere lengths. We estimate that the shortest telomeres are around 10 kb in length, indicating that each mouse cell has a few telomeres with (T(2)AG(3))(n) lengths within the range of human telomeres. These short telomeres may be critical in limiting the replicative potential of murine cells.