Rapid H1 linker histone transitions following fertilization or somatic cell nuclear transfer: evidence for a uniform developmental program in mice
Rapid H1 linker histone transitions following fertilization or somatic cell nuclear transfer: evidence for a uniform developmental program in mice
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DOI：
10.1016/j.ydbio.2003.10.003
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发表时间：
2004-02-01
影响因子：
2.7
通讯作者：
Latham, KE
中科院分区：
文献类型：
--
作者：
Gao, SR;Chung, YG;Latham, KE
H1连接组蛋白（H1s）是染色质结构和功能的关键调节因子。不同H1在早期胚胎发生过程中的功能以及调节其与染色质结合的机制在很大程度上尚不清楚。研究了H1s在卵母细胞生长和成熟、受精和早期胚胎发生以及克隆胚胎中的发育转变。卵母细胞特异性H1FOO，而不是体细胞H1s，与卵母细胞（生长，GV期和MII停滞），原核和极体中的染色质相关。H1FOO与精子或体细胞染色质在5分钟内的卵胞浆内单精子注射（ICSI）或体细胞核移植（SCNT），并完全取代体细胞H1s的60分钟。从体细胞H1s的切换到H1FOO以下SCNT发育调节。H1FOO被替换为体细胞H1s在后期的两个和四个细胞阶段。H1FOO与染色质的结合可以在核被膜存在的情况下发生，并且独立于原核的形成，由与纺锤体相关的因子调节，并且可能是一个活跃的过程。所有SCNT构建体都重现了H1转换的正常序列，表明仅此并不意味着高发育潜力。在两细胞胚胎中缺乏所有已知的H1可能有助于受精胚胎中的早熟基因转录，以及克隆胚胎中体细胞特征的形成。(C)2003年爱思唯尔公司All rights reserved.
H1 linker histones (H1s) are key regulators of chromatin structure and function. The functions of different H1s during early embryogenesis, and mechanisms regulating their associations with chromatin are largely unknown. The developmental transitions of H1s during oocyte growth and maturation, fertilization and early embryogenesis, and in cloned embryos were examined. Oocyte-specific H1FOO, but not somatic H1s, associated with chromatin in oocytes (growing, GV-stage, and MII-arrested), pronuclei, and polar bodies. H1FOO associated with sperm or somatic cell chromatin within 5 min of intracytoplasmic sperm injection (ICSI) or somatic cell nuclear transfer (SCNT), and completely replaced somatic H1s by 60 min. The switching from somatic H1s to H1FOO following SCNT was developmentally regulated. H1FOO was replaced by somatic H1s during the late two- and four-cell stages. H1FOO association with chromatin can occur in the presence of a nuclear envelope and independently of pronucleus formation, is regulated by factors associated with the spindle, and is likely an active process. All SCNT constructs recapitulated the normal sequence of H1 transitions, indicating that this alone does not signify a high developmental potential. A paucity of all known H1s in two-cell embryos may contribute to precocious gene transcription in fertilized embryos, and the elaboration of somatic cell characteristics in cloned embryos. (C) 2003 Elsevier Inc. All rights reserved.