Making medical devices safer: impact of plastic and silicone oil on microbial biofilm formation
Making medical devices safer: impact of plastic and silicone oil on microbial biofilm formation
复制标题
DOI：
10.1016/j.jhin.2020.07.011
复制
发表时间：
2020-09-01
影响因子：
6.9
通讯作者：
Brauner, A.
中科院分区：
文献类型：
--
作者：
Slettengren, M.;Mohanty, S.;Brauner, A.
背景：医疗器械面临着附着在表面的微生物生物膜的挑战。最终，这可能会危及器械的功能，并增加患者感染的风险。目的：研究硅油涂层聚丙烯塑料用于新型自动尿量计对生物膜形成的影响，探讨硅油粘度对生物膜形成的影响，并与聚苯乙烯（另一种常用的医用塑料）进行比较。常见的病原体，包括产超广谱β-内酰胺酶（ESBL）和多重耐药菌，以及白色念珠菌，进行了调查。使用缺乏重要生物膜形成因子curli、纤维素和1型菌毛（fim D）的同基因大肠杆菌菌株来确定硅油的可能作用模式。用低粘度或中粘度硅油预处理透明平底聚丙烯或聚苯乙烯威尔斯孔，并加入微生物。72 h后，使用结晶紫assay.Findings定量生物膜形成：硅油涂覆的聚丙烯塑料表面，无论油的粘度，显着抑制所有测试的革兰氏阴性和革兰氏阳性细菌，包括产ESBL和多重耐药菌株，以及C.白色念珠菌。硅油不影响细菌或念珠菌生长，卷曲菌毛是硅油的主要靶点。结论：本研究为减少微生物生物被膜的形成提供了一种新的策略，可减少医院获得性感染，预防医疗器械的功能障碍。(C)2020年，任作家。由Elsevier Ltd代表The Healthcare Infection Society出版。
Background: Medical devices face the challenge of microbial biofilm attached to the surface. Ultimately, this may jeopardize the function of the device and increase the patient's risk of infection. However, reliable methods to prevent biofilm are lacking.Aim: To investigate the effect of silicone oil-coated polypropylene plastic, used in a new automatic urinometer, on biofilm formation; furthermore, to explore the impact of silicone oil viscosity and compare polypropylene with polystyrene, another common medical plastic.Methods: Common pathogens, including extended-spectrum beta lactamase (ESBL)-producing and multi-drug-resistant bacteria, as well as Candida albicans, were investigated. Isogenic Escherichia coli strains deficient in the important biofilm forming factors curli, cellulose and type 1 fimbriae (fim D) were used to determine the possible mode of action by silicone oil. Clear flat-bottomed polypropylene or polystyrene wells were pretreated with either low- or medium-viscosity silicone oil and microbes were added. After 72 h, biofilm formation was quantified using crystal violet assay.Findings: Silicone oil-coated polypropylene plastic surfaces, regardless of the oil viscosity, significantly inhibited biofilm formation of all tested Gram-negative and Gram-positive bacteria, including ESBL-producing and multi-drug resistant strains, as well as C. albicans. Silicone oil did not affect bacterial or candida growth and curli fimbriae were found to be the main target of silicone oil. Polypropylene plastic itself without oil had a better effect in preventing biofilm formation than polystyrene.Conclusion: These findings suggest a new strategy to decrease microbial biofilm formation, which may reduce hospital-acquired infections and prevent dysfunction of medical devices. (C) 2020 The Authors. Published by Elsevier Ltd on behalf of The Healthcare Infection Society.