Hydrogen-rich saline protects against intestinal ischemia/reperfusion injury in rats
Hydrogen-rich saline protects against intestinal ischemia/reperfusion injury in rats
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DOI：
10.1080/10715760902870603
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发表时间：
2009-01-01
影响因子：
3.3
通讯作者：
Yuan, Hongbin
中科院分区：
文献类型：
--
作者：
Zheng, Xingfeng;Mao, Yanfei;Yuan, Hongbin
据报道，氢气可以减少活性氧，减轻脑、心肌和肝脏缺血/再灌注（I/R）损伤。本文研究了较易临床应用的富氢盐水对肠I/R损伤的影响。建立雄性sd大鼠肠I/R损伤模型。再灌注前10分钟静脉滴注生理盐水、富氢盐水或富氮盐水（5 ml/kg）。I/R损伤后，采用镜下观察和Chiu评分法评估小肠损伤。I/R损伤后血清DAO活性、TNF-、IL-1和IL-6水平、组织MDA、蛋白羰基和MPO活性均显著升高。富氢盐水降低了这些标记物，减轻了肠道形态学损伤，而富氮盐水处理的动物没有明显减少。综上所述，富氢盐水可能通过减少炎症和氧化应激来保护小肠免受I/R损伤。
Hydrogen gas was reported to reduce reactive oxygen species and alleviate cerebral, myocardial and hepatic ischemia/reperfusion (I/R) injuries. This paper studied the effect of hydrogen-rich saline, which was easier for clinical application, on the intestinal I/R injury. Model of intestinal I/R injury was induced in male Sprague-Dawley rats. Physiological saline, hydrogen-rich saline or nitrogen-rich saline (5 ml/kg) was administered via intravenous infusion at 10 min before reperfusion, respectively. The intestine damage was detected microscopically and was assessed by Chiu score system after I/R injury. In addition, serum DAO activity, TNF-, IL-1 and IL-6 levels, tissue MDA, protein carbonyl and MPO activity were all increased significantly by I/R injury. Hydrogen-rich saline reduced these markers and relieved morphological intestinal injury, while no significant reduction was observed in the nitrogen-rich saline-treated animals. In conclusion, hydrogen-rich saline protected the small intestine against I/R injury, possibly by reduction of inflammation and oxidative stress.