Analysis of the DNA translocation and unwinding activities of T4 phage helicases.
Analysis of the DNA translocation and unwinding activities of T4 phage helicases.
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T4 噬菌体解旋酶的 DNA 易位和解旋活性分析。
DOI：
10.1016/j.ymeth.2010.02.011
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发表时间：
2010
期刊：
影响因子：
--
通讯作者：
Benkovic,StephenJ
中科院分区：
文献类型：
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作者：
Perumal,SenthilK;Raney,KevinD;Benkovic,StephenJ
解旋酶是参与 DNA 和 RNA 代谢的一类重要酶，可耦合 ATP 水解的能量以解开双链 DNA 和 RNA 结构。了解解旋酶的作用机制至关重要，因为它们参与 DNA 复制、修复和重组等各种生物过程。此外，这类酶的双链DNA解旋特性与其单链DNA易位密切相关。因此，对其易位特性的研究对于了解解旋酶活性至关重要。在这里，我们回顾了用于分析噬菌体 T4 UvsW 和 Dda 解旋酶的 DNA 易位和解旋活性的方法。这些方法已成功用于研究大超家族的解旋酶的功能。
Helicases are an important class of enzymes involved in DNA and RNA metabolism that couple the energy of ATP hydrolysis to unwind duplex DNA and RNA structures. Understanding the mechanism of helicase action is vital due to their involvement in various biological processes such as DNA replication, repair and recombination. Furthermore, the duplex DNA unwinding property of this class of enzymes is closely related to their single-stranded DNA translocation. Hence the study of its translocation properties is essential to understanding helicase activity. Here we review the methods that are employed to analyze the DNA translocation and unwinding activities of the bacteriophage T4 UvsW and Dda helicases. These methods have been successfully employed to study the functions of helicases from large superfamilies.