Trophoblast cell lineage in cloned mouse embryos
Trophoblast cell lineage in cloned mouse embryos
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DOI：
10.1111/j.1440-169x.2010.01173.x
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发表时间：
2010-03
期刊：
影响因子：
4.6
通讯作者：
M. Oda;K. Shiota;Satoshi Tanaka
中科院分区：
文献类型：
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作者：
M. Oda;K. Shiota;Satoshi Tanaka
大多数通过小鼠体细胞核移植（SCNT）获得的胚胎由于胚胎或胚胎外缺陷而发生发育停滞。即使胎儿存活到足月，也经常出现明显的胎盘过度生长或胎盘肥大，这表明SCNT影响滋养细胞谱系的发育。滋养细胞系是在囊胚阶段建立的，滋养细胞系的干细胞群位于极滋养外胚层。因此，SCNT伴随的发育停滞和胎盘增大可能是由于供体体细胞核重编程不足导致的，无法使细胞获得滋养细胞系干细胞的完全效力。尽管SCNT胚胎的胚胎外组织存在异常，但从SCNT囊胚中已成功分离出滋养细胞干（TS）细胞系，其特性与来自天然囊胚的TS细胞没有区别。这表明SCNT不影响TS细胞的出现和自主特性。在本文中，我们讨论了SCNT囊胚中TS细胞的细胞谱系和重编程的程度。
Most conceptuses derived by somatic cell nuclear transfer (SCNT) in mice undergo developmental arrest as a result of embryonic or extraembryonic defects. Even when fetuses survive to term, prominent placental overgrowth or placentomegaly is often present, indicating that SCNT affects the development of trophoblast cell lineage. The trophoblast cell lineage is established at the blastocyst stage when the stem cell population of the trophoblast cell lineage resides in the polar trophectoderm. Therefore, it is possible that the developmental arrest and placentomegaly that accompany SCNT are induced by insufficient reprogramming of the donor somatic nucleus to enable the cells to acquire full potency as stem cells of the trophoblast cell lineage. Despite the abnormalities of the extraembryonic tissues of SCNT embryos, trophoblast stem (TS) cell lines have been successfully isolated from SCNT blastocysts and their properties appear to be indistinguishable from those of TS cells derived from native blastocysts. This suggests that SCNT does not affect the emergence and autonomous properties of TS cells. In this review, we discuss specification of cell lineage and the extent of reprogramming of TS cells in SCNT blastocysts.