Immunohistochemical profile of galectin-8 expression in benign and malignant tumors of epithelial, mesenchymatous and adipous origins, and of the nervous system
Immunohistochemical profile of galectin-8 expression in benign and malignant tumors of epithelial, mesenchymatous and adipous origins, and of the nervous system
复制标题
DOI：
10.14670/hh-16.861
复制
发表时间：
2001-07-01
影响因子：
2
通讯作者：
Kiss, R
中科院分区：
文献类型：
--
作者：
Danguy, A;Rorive, S;Kiss, R
本研究旨在探讨Galectin-8在不同组织来源的肿瘤组织中的表达是否可作为诊断标记物，包括上皮性(n=145)、间叶性(n=16)、脂肪(n=10)和中枢及周围神经系统(n=25)组织，以及4例间皮瘤。用抗Galectin-8多克隆抗体和取自布鲁塞尔伊拉斯谟大学医院解剖病理学实验室档案的组织切片进行免疫组织化学反应。本文对45例正常人、41例良性肿瘤和114例恶性肿瘤石蜡包埋组织进行了研究。免疫组化Galectin-8在结肠癌(p=0.001)、胰腺(p=0.007)、肝脏(p=0.0008)、皮肤(p=0.002)和喉癌(p=0.02)组织中的表达明显降低。在乳房组织中观察到相反的关系(p=0.007)。在肺、膀胱、肾、前列腺和胃组织中比较正常组织和/或良、恶性肿瘤时，差异无统计学意义(p>；0.05)。Galectin-8在非上皮性组织中也有显著表达，包括卵巢癌。中枢和外周神经系统以及骨骼肌和间皮瘤。因此，对Galectin-8的免疫组织化学监测揭示了在不同组织类型的上皮来源的恶性转化中，Galectin-8的表达具有器官类型依赖性的调节。这一观察结果将促使进一步的研究，以描绘任何与预后的关系。
This study aims to investigate whether the immunohistochemical expression of galectin-8 could be used as a diagnostic marker in tumor tissues of various histogenetic origins including specimens from epithelial (n=145), mesenchymatous (n=16), adipous (n=10) and central and peripheral nervous system (n=25) tissue, and 4 mesotheliomas. Immunohistochemical reactions were carried out with a polyclonal anti-galectin-8 antibody and histological slides from tissues derived from the files of the Laboratory of Anatomopathology of University Erasmus Hospital, Brussels. Formalin-fixed paraffin-embedded tissues of 45 normal cases as well as 41 benign and 114 malignant tumors were studied. Marked decreases in immunohistochemical galectin-8 expression were observed in colon (p=0.001), pancreas (p=0.007), liver (p=0.0008), skin (p=0.002) and larynx (p=0.02) tissue when comparing malignant tissue to normal tissue and/or benign tumors. The reverse relationship was observed for breast tissue (p=0.007). No statistically significant differences (p >0.05) were detected when comparing normal tissue and/or benign to malignant tumors in lung, bladder, kidney, prostate and stomach tissue. Significant galectin-8 expression was also measured in non-epithelial tissue including tumors of the. central and peripheral nervous system as well as in skeletal muscle and mesotheliomas. Immunohistochemical monitoring of galectin-8 thus reveals an organ-type-dependent regulation of expression upon malignant transformation of various tissue types of epithelial origin. This observation will prompt further studies to delineate any relationship with prognosis.