The genetic organization of arginine biosynthesis in Pseudomonas aeruginosa
The genetic organization of arginine biosynthesis in Pseudomonas aeruginosa
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铜绿假单胞菌精氨酸生物合成的遗传组织
DOI：
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发表时间：
1977
期刊：
影响因子：
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通讯作者：
T. Leisinger
中科院分区：
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作者：
D. Haas;B. Holloway;A. Schamboeck;T. Leisinger
摘要通过酶测定鉴定了铜绿假单胞菌 PAO 菌株中编码精氨酸生物合成酶的 6 个位点：argA（N-乙酰谷氨酸合成酶）、argB（N-乙酰谷氨酸 5-磷酸转移酶）、argC（N-乙酰谷氨酸 5-半醛脱氢酶）、argF（合成代谢鸟氨酸氨基甲酰转移酶）、argG （精氨基琥珀酸合成酶）和 argH（精氨基琥珀酸酶）。需要精氨酸和尿嘧啶的一步突变体在氨基甲酰磷酸合酶中存在缺陷，由指定的基因座 car 指定。为了绘制这些突变图谱，我们在改进的中断交配技术中使用了性别因子 FP2 以及通用转导噬菌体 F116L 和 G101。我们证实了早期的研究，没有发现 arg 和 car 基因座的聚集。然而，argA、argH 和 argB 被定位在短染色体片段（大约 3 分钟长）上，argF 和 argG 是可共转导的，但不连续。
N-乙酰谷氨酸合酶是补充假单胞菌鸟氨酸合成中乙酰化中间体循环的酶，似乎对于精氨酸合成至关重要，因为 argA 突变体在未补充的基本培养基上不生长。
SummarySix loci coding for arginine biosynthetic enzymes in Pseudomonas aeruginosa strain PAO were identified by enzyme assay: argA (N-acetylglutamate synthase), argB (N-acetylglutamate 5-phosphotransferase), argC (N-acetylglutamate 5-semialdehyde dehydrogenase), argF (anabolic ornithine carbamoyltransferase), argG (argininosuccinate synthetase), and argH (argininosuccinase). One-step mutants which had a requirement for arginine and uracil were defective in carbamoylphosphate synthase, specified by a locus designated car. To map these mutations we used the sex factor FP2 in an improved interrupted mating technique as well as the generalized transducing phages F116L and G101. We confirmed earlier studies, and found no clustering of arg and car loci. However, argA, argH, and argB were mapped on a short chromosome segment (approx. 3 min long), and argF and argG were cotransducible, but not contiguous.
N-Acetylglutamate synthase, the enzyme which replenishes the cycle of acetylated intermediates in ornithine synthesis of Pseudomonas, appears to be essential for arginine synthesis since argA mutants showed no growth on unsupplemented minimal medium.