Photochemical Inactivation of Bacteria and HIV in Buffy–Coat–Derived Platelet Concentrates under Conditions That Preserve in vitro Platelet Function
Photochemical Inactivation of Bacteria and HIV in Buffy–Coat–Derived Platelet Concentrates under Conditions That Preserve in vitro Platelet Function
复制标题
在保留体外血小板功能的条件下，光化学灭活血沉棕黄层来源的血小板浓缩物中的细菌和 HIV
作者：
F. Knutson;R. Alfonso;K. Dupuis;V. Mayaudon;Lily Lin;L. Corash;C. Högman
背景和目的：已经测试了一种光化学方法来灭活淡黄色涂层衍生的血小板浓缩物(BC PC)中的病毒和细菌。材料和方法：用15 0μM补骨脂素S 59和3J/cm2的长波紫外线(UVA，32 0~40 0 nm)照射35%CPD血浆和6 5%血小板添加剂溶液(PAS III)中的BC PC。在PCT后，使用一组体外检测来评估血小板功能。结果：该PCT工艺对革兰氏阳性菌(表皮葡萄球菌、金黄色葡萄球菌、粪肠球菌)和革兰氏阴性菌(产气肠杆菌、铜绿假单胞菌、粘质沙雷氏菌)均有较好的灭活效果。在PCT和血小板保存7天后，没有检测到活细菌，而在配对的未经处理的对照BC PC中检测到细菌生长。完全灭活革兰氏阳性蜡状芽孢杆菌只在BC PC的两个重复实验中的一个中实现。PCT对接种约106μ组织培养感染量/毫升(TCID50/ml)的BCPC中的HIV-1也有很高的灭活效果。随着UVA剂量的增加，灭活迅速：150μM S-59和1J/cm~2 UVA处理，可达到5.6±0.5logTCID50/ml的减少，150 Log S-59和3J/cm~2 UVA处理可达到减少6.4logTCID50/ml的效果。在7天的储存期间，测试和对照BC PC之间的血小板功能没有发现生理上的差异。结论：含15 0μM S 59的PCT和3J/cm2的UVA处理对22℃保存7d的BC PC的体外性能无不良影响。PCT过程将灭活细菌和HIV-1接种到BC PC中。这些结果将早先报道的PCT分离PC的有效性扩展到BC PC。
Background and Objectives: A photochemical process has been tested for the inactivation of viruses and bacteria in buffy–coat derived platelet concentrates (BC PCs). Materials and Methods: BC PCs in 35% CPD plasma and 65% platelet–additive solution (PAS III) were exposed to photochemical treatment (PCT) with 150 μM of the psoralen S–59 and a 3 J/cm2 treatment with long–wavelength ultraviolet light (UVA, 320–400 nm). Platelet function was evaluated following PCT using a panel of in vitro assays. Results: This PCT process was highly effective at inactivating gram–positive bacteria (Staphylococcus epidermidis, Staphylococcus aureus, Enterococcus faecalis) and gram–negative bacteria (Enterobacter aerogenes, Pseudomonas aeruginosa, Serratia marcescens). No viable bacteria were detected following PCT and 7 days of platelet storage while bacterial growth was detected in paired untreated control BC PCs. Complete inactivation of the gram–positive Bacillus cereus was achieved only in one of two replicate experiments with BC PCs. PCT was also highly effective for inactivation of human immunodeficiency virus HIV–1 in BC PCs inoculated with approximately 106 tissue culture infectious doses per milliliter (TCID50/ml) of cell–associated HIV–1. Rapid inactivation was observed with increasing UVA doses: with 150 μM S–59 and a 1 J/cm2 treatment of UVA, a reduction of 5.6±0.5 log TCID50/ml was achieved, and a reduction of >6.4 log TCID50/ml was achieved with 150 μM S–59 and a 3 J/cm2 treatment of UVA. No physiologically relevant differences in platelet functions were found between the test and the control BC PCs during 7 days of storage. Conclusion: PCT with 150 μM S–59 and a 3 J/cm2 UVA treatment does not adversely affect in vitro properties of BC PCs stored at 22°C for 7 days. The PCT process inactivated bacteria and HIV–1 inoculated into the BC PCs. These results extend the earlier reported efficacy of PCT apheresis PCs to BC PCs.
影响因子：
20.3
作者：
J. Grass;Tamim Wafa;A. Reames;D. Wages;L. Corash;J. Ferrara;Lily Lin
通讯作者：
J. Grass;Tamim Wafa;A. Reames;D. Wages;L. Corash;J. Ferrara;Lily Lin
人血小板浓缩物中致病菌的光化学灭活。
影响因子：
20.3
作者：
Lin,L;Londe,H;Janda,JM;Hanson,CV;Corash,L
通讯作者：
Corash,L