SELECTIVE CLEAVAGE OF 28S RIBOSOMAL-RNA VARIABLE REGIONS V3 AND V13 IN MYELOID-LEUKEMIA CELL APOPTOSIS
SELECTIVE CLEAVAGE OF 28S RIBOSOMAL-RNA VARIABLE REGIONS V3 AND V13 IN MYELOID-LEUKEMIA CELL APOPTOSIS
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DOI：
10.1016/0014-5793(93)81123-h
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发表时间：
1993-01-02
期刊：
影响因子：
3.5
通讯作者：
LANOTTE, M
中科院分区：
文献类型：
--
作者：
HOUGE, G;DOSKELAND, SO;LANOTTE, M
在大鼠髓性白血病细胞系IPC-81中cAMP依赖性蛋白激酶I活化后3-4小时诱导了强烈的细胞凋亡[J. Cell. 146（1991）73-80]。我们将报告这些细胞中凋亡的一个新特征：两个最大的28 S核糖体RNA可变区（V3和V13）内的选择性和暂时有序的裂解。28 S rRNA的裂解与核小体间DNA断裂和细胞蛋白质合成的停止相一致。28 S可变区作为凋亡靶点的意义是真核核糖体这些迄今为止明显多余部分功能的线索。
Vigorous apoptosis is induced 3-4 hours after activation of cAMP-dependent protein kinase I in the rat myeloid leukemia cell line IPC-81 [J. Cell. Physiol. 146 (1991) 73-80]. We will report a novel feature of apoptosis in these cells: a selective and temporarily ordered cleavage within the two largest 28S ribosomal RNA variable regions (V3 and V13). The cleavage of 28S rRNA coincided with internucleosomal DNA fragmentation and cessation of cellular protein synthesis. The implication of 28S variable regions as targets in apoptosis is a clue to the function of these so far apparently superfluous parts of eukaryotic ribosomes.