Development of co-dominant KASP markers co-segregating with Ug99 effective stem rust resistance gene Sr26 in wheat
Development of co-dominant KASP markers co-segregating with Ug99 effective stem rust resistance gene Sr26 in wheat
复制标题
DOI：
10.1007/s11032-018-0854-6
复制
发表时间：
2018-07
影响因子：
3.1
通讯作者：
Naeela Qureshi;P. Kandiah;M. Gessese;V. Nsabiyera;V. Wells;P. Babu;Debbie Wong;M. Hayden;H. Bariana;U. Bansal
中科院分区：
文献类型：
--
作者：
Naeela Qureshi;P. Kandiah;M. Gessese;V. Nsabiyera;V. Wells;P. Babu;Debbie Wong;M. Hayden;H. Bariana;U. Bansal
小麦茎锈病是由小麦锈菌引起的。小麦根结线虫(sp.tritici，PGT)是一种危害全球粮食安全的细菌，它能导致作物全面歉收。在东非检测到的PGT小种TTKSK(UG99)及其衍生物对现代栽培品种中存在的许多抗性基因具有毒力。然而，抗茎锈病基因Sr26对世界上所有的PGT小种都有效，其携带于长穗冰草(Thinopyrum Ponticum)的6Ae#1L易位到6AL染色体上。本研究以地方品种Aus27969与Avocet S杂交的重组自交系群体为材料，构建了与Sr26共分离的共显性等位基因特异性聚合酶链式反应(Kasp)标记。4个KASP标记(sunKASP_216、sunKASP_218、sunKASP_224和sunKASP_225)在另外4个RIL群体中与Sr26共分离。在澳大利亚品种和育种系上测试时，这些标记在所有已知缺乏Sr26基因的品种中扩增出与sr26连锁的等位基因，在已知携带sr26的品种和基因型中扩增出与sr26连锁的等位基因。携带最短SR26易位片段的Wa-1和Wa-1/3*Yitpi仅对标记sunKasp_224和sunKasp_225呈阳性。我们的结果表明，4个KASP标记位于原始易位上，SunKASP_224和SunKASP_225位于缩短易位上。因此，sunKasp_224和sunKasp_225可用于SR26与其他抗茎锈病基因的标记辅助聚合，以获得持久抗性。
Stem rust of wheat, caused byPuccinia graminisf. sp.tritici(Pgt), is a threat to global food security due to its ability to cause total crop failures. The Pgt race TTKSK (Ug99) and its derivatives detected in East Africa carry virulence for many resistance genes present in modern cultivars. However, stem rust resistance geneSr26remains effective to all races of Pgt worldwide.Sr26is carried on theAgropyron elongatum(syn.Thinopyrum ponticum) segment 6Ae#1L translocated to chromosome 6AL of wheat. In this study, a recombinant inbred line (RIL) population derived from a cross between the landrace Aus27969 and Avocet S, which carriesSr26, was used to develop co-dominant kompetitive allele-specific polymerase chain reaction (KASP) markers that co-segregate withSr26. Four KASP markers (sunKASP_216,sunKASP_218,sunKASP_224andsunKASP_225) were also shown to co-segregate withSr26in four additional RIL populations. When tested on Australian cultivars and breeding lines, these markers amplified alleles alternate to that linked withSr26in all cultivars known to lack this gene andSr26-linked alleles in cultivars and genotypes known to carrySr26. Genotypes WA-1 and WA-1/3*Yitpi carrying the shortestSr26translocation segment were positive only for markerssunKASP_224andsunKASP_225. Our results suggest the four KASP markers are located on the original translocation andsunKASP_224andsunKASP_225are located on the shortened version. Therefore,sunKASP_224andsunKASP_225can be used for marker-assisted pyramiding ofSr26with other stem rust resistance genes to achieve durable resistance in wheat.