Generation of transgenic frogs.
Generation of transgenic frogs.
复制标题
DOI：
10.1007/978-1-60327-019-9_4
复制
发表时间：
2009-01-01
期刊：
影响因子：
--
通讯作者：
Pieler, Tomas
中科院分区：
文献类型：
--
作者：
Loeber, Jana;Pan, Fong Cheng;Pieler, Tomas
获得转基因动物的可能性对于分析基因在发育和疾病中的功能具有明显的优势。两栖动物非洲爪蟾是发育生物学中已建立的脊椎动物模型系统之一。不同的技术已经成功地应用于创建非洲爪蟾转基因;在本章中，所谓的大范围核酸酶的方法进行了描述。该技术不仅技术简单，而且高效，适用于非洲爪蟾和热带爪蟾。商业上可获得的内切核酸酶I-SceI（大范围核酸酶）在共注射到受精卵中后介导外源DNA整合到青蛙基因组中。已经观察到组织特异性基因表达以及生殖系传播。
The possibility of generating transgenic animals is of obvious advantage for the analysis of gene function in development and disease. One of the established vertebrate model systems in developmental biology is the amphibian Xenopus laevis. Different techniques have been successfully applied to create Xenopus transgenics; in this chapter, the so-called meganuclease method is described. This technique is not only technically simple, but also comparably efficient and applicable to both Xenopus laevis and Xenopus tropicalis. The commercially available endonuclease I-SceI (meganuclease) mediates the integration of foreign DNA into the frog genome after coinjection into fertilized eggs. Tissue-specific gene expression, as well as germline transmission, has been observed.