Comparison between Stem Cells Harvested from Wet and Dry Lipoaspirates
Comparison between Stem Cells Harvested from Wet and Dry Lipoaspirates
复制标题
DOI：
10.3109/03008207.2012.717130
复制
发表时间：
2013-01-01
影响因子：
2.9
通讯作者：
Morselli, Paolo Giovanni
中科院分区：
文献类型：
--
作者：
Muscari, Claudio;Bonafe, Francesca;Morselli, Paolo Giovanni
脂肪干细胞（ASC）通常从脂肪抽吸物中分离出来，但尚不清楚注射到脂肪组织中的麻醉溶液是否会影响细胞产量和功能。从女性受试者的腹部区域抽取了两个不同的样本。首先，注射含有利多卡因/肾上腺素的生理溶液（湿式吸脂，WL），而在对侧区域，在不注射任何溶液的情况下收集样本（干式吸脂，DL）。对抽吸物进行处理以研究基质血管部分 (SVF) 细胞的产量以及 ASC 频率、生长速率、细胞凋亡和分化潜力。新鲜 WL 分离株的固体干燥质量低于 DL 分离株 (p < 0.01)，因为前者存在液体溶液。结果，WL-SVF 细胞的数量比 DL 获得的细胞数量低 18.7% (p < 0.01)；在培养条件下也观察到这种差异。此外，从 1 x 10(3) SVF 细胞中获得的集落形成单位成纤维细胞 (CFU-F) 的数量在 WL 抽吸物中比 DL 抽吸物低 25.5% (p < 0.05)，这至少部分是由于观察到 WL 分离物的液体溶液中存在表观扩散系数。 WL 和 DL 后，未观察到 ASC 生长速率、细胞凋亡或向脂肪形成、成骨和内皮细胞谱系的分化潜力存在差异。总之，由于组织稀释和 SVF 中 ASC 频率降低的综合作用，WL 产生的 ASC 比 DL 少约 40%。 ASC的主要生物学特征适合细胞疗法。
Adipose-derived stem cells (ASC) are usually isolated from lipoaspirates, but it is not known if the anesthetic solution injected into adipose tissue affects cell yield and functions. Two different samples were drawn from the abdominal region of female subjects. In the first, a physiological solution containing lidocaine/adrenaline was injected (wet liposuction, WL), while in the contralateral area, the sample was collected without injecting any solution (dry liposuction, DL). The aspirates were processed to investigate the yield of the stromal-vascular fraction (SVF) cells and ASC frequency, growth rate, apoptosis, and differentiation potential. The solid dried mass of fresh WL isolates was lower than that of DL isolates (p < 0.01) due to the presence, in the former, of a liquid solution. As a consequence, the amount of WL-SVF cells was 18.7% lower than those obtained from DL (p < 0.01); this difference was also observed under culture conditions. In addition, the number of colony-forming unit-fibroblasts (CFU-Fs) obtained from 1 x 10(3) SVF cells was 25.5% lower in WL-aspirates than DL-aspirates (p < 0.05) owing, at least in part, to the observed presence of apparent diffusion coefficient in the liquid solution of the WL isolates. After WL and DL, no differences were observed in ASC growth rate, apoptosis, or differentiation potential toward adipogenic, osteogenic, and endothelial cell lineages. In conclusion, WL yields about 40% fewer ASC than DL due to the combined effect of tissue dilution and the reduced frequency of ASC in the SVF. The main biological features of ASC are suitable for cell-based therapies.