LACTATE-SUPPORTED SYNAPTIC FUNCTION IN THE RAT HIPPOCAMPAL SLICE PREPARATION
LACTATE-SUPPORTED SYNAPTIC FUNCTION IN THE RAT HIPPOCAMPAL SLICE PREPARATION
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DOI：
10.1126/science.3375817
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发表时间：
1988-06-03
期刊：
影响因子：
56.9
通讯作者：
RIGOR, BM
中科院分区：
文献类型：
--
作者：
SCHURR, A;WEST, CA;RIGOR, BM
本研究旨在探讨乳酸对脑能量代谢的促进作用。作为唯一的能量底物，乳酸支持大鼠海马切片的正常突触功能数小时，没有任何恶化的迹象。由于葡萄糖耗尽而导致突触沉默的切片可以用乳酸盐重新激活，以显示正常的突触功能。当切片暴露于糖酵解抑制剂碘乙酸时，乳酸盐支持的突触功能不受影响，而葡萄糖支持的突触功能被完全废除。这表明乳酸直接通过丙酮酸代谢进入三羧酸循环。因此，在导致乳酸积累（脑缺血）的条件下，这种“终产物”可能是一种有用的替代品，作为能量代谢的底物。
The present study was undertaken to examine the possibility that cerebral energy metabolism can be fueled by lactate. As a sole energy substrate, lactate supported normal synaptic function in rat hippocampal slices for hours without any sign of deterioration. Slices that were synaptically silent as a results of glucose depletion could be reactivated with lactate to show normal synaptic function. When slices were exposed to the glycolytic inhibitor iodoacetic acid, lactate-supported synaptic function was unaffected, whereas that supported by glucose was completely abolished. This indicated that lactate was metabolized directly via pyruvate to enter the tricarboxylic acid cycle. Thus, under conditions that lead to lactate accumulation (cerebral ischemia) this "end product" may be a useful alternative as a substrate for energy metabolism.