A and B forms of the androgen receptor are present in human genital skin fibroblasts.
A and B forms of the androgen receptor are present in human genital skin fibroblasts.
复制标题
雄激素受体的 A 型和 B 型存在于人类生殖器皮肤成纤维细胞中。
DOI：
10.1073/pnas.91.4.1234
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发表时间：
1994
影响因子：
11.1
通讯作者：
McPhaul,MJ
中科院分区：
文献类型：
--
作者：
Wilson,CM;McPhaul,MJ
两种形式的雄激素受体（AR）蛋白（表观分子量，约110 kDa和约87 kDa）存在于人类生殖器皮肤成纤维细胞。87-kDa亚型（AR-A）含有完整的C末端，但缺乏110-kDa亚型（AR-B）中发现的正常N末端。AR-A的大小与雄激素抗性个体（R776）的成纤维细胞中通过AR-B内部Met-188残基开始AR合成产生的AR突变体相同。来源于正常个体的成纤维细胞中AR-B与AR-A的比例约为10：1。在这些实验中检测到的AR同种型类似于人孕酮受体的A和B型，其也由单个基因编码，不同之处在于N-末端片段的存在或缺失。人孕酮受体的A和B型在其激活靶基因的能力上不同，并且在各种类型的细胞中受到不同的调节。人类AR的相似形式的鉴定提出了AR-A和AR-B也具有不同功能的可能性。
Two forms of the androgen receptor (AR) protein (apparent molecular masses, approximately 110 kDa and approximately 87 kDa) are present in human genital skin fibroblasts. The 87-kDa isoform (AR-A) contains an intact C terminus but lacks the normal N terminus found in the 110-kDa isoform (AR-B). AR-A is the same size as the mutant form of AR produced in fibroblasts from an androgen-resistant individual (R776) by initiation of AR synthesis at the internal Met-188 residue of AR-B. The ratio of AR-B to AR-A in fibroblasts derived from normal individuals is approximately 10:1. The AR isoforms detected in these experiments resemble the A and B forms of the human progesterone receptor, which also are encoded by a single gene and differ by the absence or presence of an N-terminal segment. The A and B forms of the human progesterone receptor differ in their ability to activate target genes and are regulated differently in various types of cells. The identification of similar forms of human AR raises the possibility that AR-A and AR-B also subserve different functions.