The same dorsal root ganglion neurons innervate uterus and colon in the rat
The same dorsal root ganglion neurons innervate uterus and colon in the rat
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DOI：
10.1097/wnr.0b013e32801231bf
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发表时间：
2007-02-12
期刊：
影响因子：
1.7
通讯作者：
Micevych, Paul
中科院分区：
文献类型：
--
作者：
Chaban, Victor;Christensen, Amy;Micevych, Paul
本研究的目的是确定支配子宫的初级感觉神经元是否接收来自结肠的汇聚输入。为了测试这一点，在大鼠中，用荧光示踪染料逆行标记结肠和子宫背根神经节的细胞体，并将其显微注射到结肠/直肠和双侧子宫角中。收获神经节、冷冻保护并切成20μm切片以鉴定用于荧光显微镜检查的阳性染色细胞。高达 5% 的神经元是结肠特异性或子宫特异性的，10-15% 的标记神经节神经元在 L-1、L-2、L-6 和 S1-S3 水平上支配两个内脏。这些结果表明背根神经节内脏感觉统合的一种新形式可能是许多功能性疼痛综合征合并症的基础。
The purpose of this study was to determine whether primary sensory neurons that innervate the uterus receive convergent input from the colon. To test this, in the rat, cell bodies of colonic and uterine dorsal root ganglia were retrogradely labeled with fluorescent tracer dyes microinjected into the colon/rectum and bilaterally into the uterine horns. Ganglia were harvested, cryoprotected and cut into 20 mu m slices to identify positively stained cells for fluorescent microscopy. Up to 5% of neurons were colon-specific or uterus-specific, and 10-15% of labeled ganglion neurons innervate both viscera in the L-1, L-2, L-6 and S1-S3 levels. These results suggest a novel form of visceral sensory integration in the dorsal root ganglion that may underlie comorbidity of many functional pain syndromes.