Cross talk between T and B cells generates B antigen-presenting cells able to induce inositol phosphate production in T cells responding to Mls(a) superantigens.
Cross talk between T and B cells generates B antigen-presenting cells able to induce inositol phosphate production in T cells responding to Mls(a) superantigens.
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T 细胞和 B 细胞之间的串扰产生 B 抗原呈递细胞，能够诱导响应 Mls(a) 超抗原的 T 细胞产生磷酸肌醇。
DOI：
10.1002/eji.1830271224
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
Webb,SR
中科院分区：
文献类型：
--
作者：
O'Rourke,AM;Webb,SR
先前的研究表明，用小鼠乳腺肿瘤病毒编码的Mlsasophila抗原激活CD 4 +T细胞可诱导强烈的增殖反应和白细胞介素-2的产生，但未能引发典型的早期T细胞受体（TCR）介导的信号转导事件，如多磷酸肌醇（PI）水解或细胞内钙增加。在这里，我们表明，当静息B细胞用作抗原呈递细胞（APC）时，MISaantigen未能激活PI水解。相比之下，当携带Mlsa的B细胞通过暴露于脂多糖或更重要的是暴露于Mlsa反应性T细胞或抗CD 40抗体活化24小时时，细胞产生了引发容易检测的PI周转的能力。这些研究表明，对于作为APC的B细胞，某些TCR相关信号转导途径的启动可能依赖于APC的激活。这些数据表明，T细胞和静息B细胞之间的串扰足以产生感受态B APC，并导致T细胞应答中重要的信号传导途径的延迟启动。
Previous studies showed that activation of CD4+T cells with mouse mammary tumor virus‐encoded Mlsasuperantigens induces strong proliferative responses and interleukin‐2 production but fails to elicit typical early T cell receptor (TCR)‐mediated signal transduction events, such as hydrolysis of polyphosphoinositides (PI) or an increase in intracellular calcium. Here we show that the failure of MIsaantigen to activate PI hydrolysis applies when resting B cells are used as antigen‐presenting cells (APC). By contrast, when MIsa‐bearing B cells are activated for 24h by exposure to lipopolysaccharide or, more importantly, to Mlsa‐reactive T cells or anti‐CD40 antibodies the cells develop the capacity to elicit easily detectable PI turnover. These studies demonstrate that, for B cells as APC, the initiation of certain TCR‐associated signal transduction pathways can depend on activation of the APC. The data suggest that cross talk between T cells and resting B cells can suffice to generate competent B APC and lead to the delayed initiation of signaling pathways important in T cell responses.